Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China.
Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China.
J Reprod Immunol. 2022 Sep;153:103677. doi: 10.1016/j.jri.2022.103677. Epub 2022 Jul 23.
Interferon-tau (IFNτ), as an antiluteolytic factor secreted by trophoderm during the pregnancy of ruminants, actually functions by activating the IFNτ receptor 1 (IFNAR1) and IFNτ receptor 2 (IFNAR2). However, it has not been clearly understood how IFNτ-IFNAR cascade regulation processes between the embryo and uterine epithelial cells in ruminants. In this study, we found the expression and location of IFNτ in the bovine blastocysts from different production sources. IFNτ, IFNAR1 and IFNAR2 were all located in the trophoblast cells of the blastocyst. However, the fluorescence intensity of IFNAR1 was consistent with that of IFNτ. Antagonizing the expressions of IFNAR1 and IFNAR2 in embryos and co-culture with endometrial epithelium cells (EECs) reduced the expressions of Integrin αv β3, WNT7A, and ISG15 in EECs. Knocking out IFNAR1 and IFNAR2 reduce the expressions of Integrin αv β3 and WNT7A in EECs, the deletion of IFNAR2 gene has a greater impact than that of IFNAR1 gene. IFNAR1/IFNAR2 and IFNAR1/IFNAR2 EECs were co-cultured with IVF embryos, the expression of Integrin αv β3 was inhibited, and the inhibition of IFNAR1/IFNAR2 was much stronger, and the expression of WNT7A was not inhibited. The expressions of Integrin αv β3 and WNT7A did not change significantly after IFNAR1/IFNAR2 and IFNAR1/IFNAR2 co-culture with PA embryos. All of these results strongly suggest that specific activation of embryonic IFNAR1 and endometrial IFNAR2 induced by embryonic IFNτ directs normal uterine preparation for bovine early implantation.
干扰素 - tau（IFNτ）作为反溶黄体因子，在反刍动物妊娠期间由滋养层分泌，实际上通过激活 IFNτ 受体 1（IFNAR1）和 IFNτ 受体 2（IFNAR2）发挥作用。然而，目前还不清楚 IFNτ-IFNAR 级联调节过程在反刍动物胚胎和子宫上皮细胞之间是如何进行的。在本研究中，我们发现了不同来源牛胚泡中 IFNτ 的表达和定位。IFNτ、IFNAR1 和 IFNAR2 均位于胚泡的滋养层细胞中。然而，IFNAR1 的荧光强度与 IFNτ 的荧光强度一致。在胚胎中拮抗 IFNAR1 和 IFNAR2 的表达并与子宫内膜上皮细胞（EEC）共培养，降低了 EEC 中整合素 αvβ3、WNT7A 和 ISG15 的表达。敲除 IFNAR1 和 IFNAR2 降低了 EEC 中整合素 αvβ3 和 WNT7A 的表达，IFNAR2 基因的缺失比 IFNAR1 基因的缺失影响更大。IFNAR1/IFNAR2 和 IFNAR1/IFNAR2 EEC 与 IVF 胚胎共培养时，整合素 αvβ3 的表达受到抑制，IFNAR1/IFNAR2 的抑制作用更强，而 WNT7A 的表达不受抑制。IFNAR1/IFNAR2 和 IFNAR1/IFNAR2 与 PA 胚胎共培养后，整合素 αvβ3 和 WNT7A 的表达没有明显变化。所有这些结果都强烈表明，胚胎 IFNτ 特异性激活胚胎 IFNAR1 和子宫内膜 IFNAR2 指导牛早期植入的正常子宫准备。
