Institute of Chemical Industry of Forest Products, CAF, Nanjing 210042, Jiangsu Province, China; Research Institute of Forestry New Technology, CAF, Beijing 100091, China.
General Hospital of Eastern Theater Command, Nanjing 210002, Jiangsu Province, China.
Int J Biol Macromol. 2022 Sep 30;217:890-901. doi: 10.1016/j.ijbiomac.2022.07.205. Epub 2022 Jul 27.
The unsustainable antibacterial activity of ruthenium antibacterial agents is an important factor limiting their applications. This present work attempts to prepare ruthenium (Ru) coordination polymer composites with chitosan quaternary ammonium polymers (CQ) and shikimic acid (SA) through the interaction of ionic bonds and covalent bonds by microwave-assisted high-pressure homogenization methods. The prepared CQ@Ru-SA was characterized by size distribution, zeta potential, TEM, UV-vis, FTIR, XPS and XRD analyses. The coordination structure and morphology of Bridge-CQ-NH-Ru-SA were verified. The CQ@Ru-SA was well-dispersed in both the aqueous or anhydrous states. MIC and MBC, time-killing curves, biofilm formation inhibition assay, mature biofilm disruption assay, SEM, Ca mobilization assay and Ca-Mg-ATPase activity studies revealed that CQ@Ru-SA had a stronger inhibitory effect against S. aureus than CQ and showed sustained antibacterial properties in the dynamic time-killing curves. Meanwhile, CQ@Ru-SA had good antibacterial effects against S. aureus and inhibited their biofilm forming ability in a dose-dependent manner. Further studies on antibacterial mechanisms revealed that CQ@Ru-SA influenced cell membrane integrity, Ca-Mg-ATPase activity on the cell membrane and intracellular Ca levels of S. aureus. This study will provide the necessary data for the further design and development of ruthenium-based photosensitive antibacterial agents.
钌抗菌剂的抗菌活性不可持续是限制其应用的一个重要因素。本工作试图通过微波辅助高压匀相法，利用离子键和共价键的相互作用，制备壳聚糖季铵盐聚合物（CQ）和莽草酸（SA）与钌（Ru）配位聚合物复合材料。通过粒径分布、Zeta 电位、TEM、UV-vis、FTIR、XPS 和 XRD 分析对制备的 CQ@Ru-SA 进行了表征。验证了桥联 CQ-NH-Ru-SA 的配位结构和形态。CQ@Ru-SA 在水相和无水状态下均具有良好的分散性。MIC 和 MBC、时效杀菌曲线、生物膜形成抑制试验、成熟生物膜破坏试验、SEM、Ca 动员试验和 Ca-Mg-ATP 酶活性研究表明，CQ@Ru-SA 对金黄色葡萄球菌的抑制作用强于 CQ，并在动态时效杀菌曲线中表现出持续的抗菌性能。同时，CQ@Ru-SA 对金黄色葡萄球菌具有良好的抗菌作用，并呈剂量依赖性抑制其生物膜形成能力。进一步的抗菌机制研究表明，CQ@Ru-SA 影响金黄色葡萄球菌的细胞膜完整性、细胞膜上的 Ca-Mg-ATP 酶活性和细胞内 Ca 水平。本研究将为进一步设计和开发基于钌的光敏感抗菌剂提供必要的数据。
