BnaPPT1 对于油菜叶绿体发育和种子油积累是必需的。

BnaPPT1 is essential for chloroplast development and seed oil accumulation in Brassica napus.

机构信息

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; Hubei Hongshan Laboratory, Wuhan 430070, China.

出版信息

J Adv Res. 2022 Dec;42:29-40. doi: 10.1016/j.jare.2022.07.008. Epub 2022 Jul 27.

DOI:10.1016/j.jare.2022.07.008

PMID:35907629

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9788935/

Abstract

INTRODUCTION

Phosphoenolpyruvate/phosphate translocator (PPT) transports phosphoenolpyruvate from the cytosol into the plastid for fatty acid (FA) and other metabolites biosynthesis.

OBJECTIVES

This study investigated PPTs' functions in plant growth and seed oil biosynthesis in oilseed crop Brassica napus.

METHODS

We created over-expression and mutant material of BnaPPT1. The plant development, oil content, lipids, metabolites and ultrastructure of seeds were compared to evaluate the gene function.

RESULTS

The plastid membrane localized BnaPPT1 was found to be required for normal growth of B. napus. The plants grew slower with yellowish leaves in BnaA08.PPT1 and BnaC08.PPT1 double mutant plants. The results of chloroplast ultrastructural observation and lipid analysis show that BnaPPT1 plays an essential role in membrane lipid synthesis and chloroplast development in leaves, thereby affecting photosynthesis. Moreover, the analysis of primary metabolites and lipids in developing seeds showed that BnaPPT1 could impact seed glycolytic metabolism and lipid level. Knockout of BnaA08.PPT1 and BnaC08.PPT1 resulted in decreasing of the seed oil content by 2.2 to 9.1%, while overexpression of BnaC08.PPT1 significantly promoted the seed oil content by 2.1 to 3.3%.

CONCLUSION

Our results suggest that BnaPPT1 is necessary for plant chloroplast development, and it plays an important role in maintaining plant growth and promoting seed oil accumulation in B. napus.

摘要

简介

磷酸烯醇丙酮酸/磷酸载体（PPT）将磷酸烯醇丙酮酸从细胞质转运到质体中，用于脂肪酸（FA）和其他代谢物的生物合成。

目的

本研究调查了 PPT 在油料作物油菜（Brassica napus）生长和种子油生物合成中的功能。

方法

我们创建了 BnaPPT1 的过表达和突变材料。通过比较植物发育、油含量、脂质、代谢物和种子超微结构来评估基因功能。

结果

发现定位于质体膜的 BnaPPT1 对于油菜的正常生长是必需的。BnaA08.PPT1 和 BnaC08.PPT1 双突变体植物生长缓慢，叶片呈黄色。叶绿体超微结构观察和脂质分析的结果表明，BnaPPT1 在叶片的膜脂合成和叶绿体发育中起着重要作用，从而影响光合作用。此外，对发育中种子的初级代谢物和脂质的分析表明，BnaPPT1 可以影响种子糖酵解代谢和脂质水平。BnaA08.PPT1 和 BnaC08.PPT1 的敲除导致种子油含量降低 2.2%至 9.1%，而 BnaC08.PPT1 的过表达则显著促进了种子油含量提高 2.1%至 3.3%。