循环 microRNA hsa-let-7d-3p 作为人类脓毒症潜在新生物标志物的证据。

Evidence for the circulating microRNA hsa-let-7d-3p as a potential new biomarker for sepsis in human subjects.

机构信息

Department of Pulmonary and Critical Care Medicine, The Eighth Medical Center of Chinese, PLA General Hospital, No. 28 Fuxing Road, 100853, Beijing, China.

Department of Respiration, LiangXiang Hospital, Fangshan, Beijing, China.

出版信息

Eur J Med Res. 2022 Jul 30;27(1):137. doi: 10.1186/s40001-022-00763-3.

DOI:10.1186/s40001-022-00763-3

PMID:35907902

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9338616/

Abstract

BACKGROUND

Current biomarkers for the early detection of sepsis have low sensitivity and specificity. Serum microRNAs (miRNAs) have been proposed as novel noninvasive biomarkers for various diseases. The aim of the present study was to discover a novel diagnostic biomarker for sepsis in human subjects.

METHODS

miRNA expression profiling was performed using peripheral blood from three sepsis patients in the sepsis stage and improved condition stage using microarray screening. The differentially expressed miRNAs were primary validated by real-time quantitative polymerase chain reaction (RT-qPCR) in a further set of 20 sepsis patients in the sepsis stage and improved condition stage. Finally, we validated the differentially expressed miRNAs in 95 sepsis patients and 66 nonsepsis patients. The validated miRNAs and patients' clinical indictors were analysed in a multivariate logistic regression model. The diagnostic value of the changed miRNA in sepsis was determined and compared with CRP and WBC by analysing the receiver operating characteristic (ROC) curves.

RESULTS

According to the criteria, we detected 11 miRNAs regulated by the miRNA chip. RT-qPCR detection showed that the expression of hsa-let-7d-3p in sepsis patients was upregulated compared with that in nonsepsis patients. In a multiple logistic regression analysis, serum miRNA hsa-let-7d-3p was found to be an independent predictor of sepsis. Receiver operating characteristic curve (ROC) analysis showed that the area under the ROC curve of serum hsa-let-7d-3p was 0.696 [95% confidence interval (0.615, 0.778)].

CONCLUSION

The miRNA hsa-let-7d-3p was identified as a novel biomarker for the early detection of sepsis.

摘要

背景

目前用于脓毒症早期检测的生物标志物的灵敏度和特异性均较低。血清 microRNAs（miRNAs）已被提出作为各种疾病的新型非侵入性生物标志物。本研究旨在发现一种用于人类脓毒症的新型诊断生物标志物。

方法

使用微阵列筛选对处于脓毒症阶段和改善阶段的 3 例脓毒症患者的外周血进行 miRNA 表达谱分析。通过实时定量聚合酶链反应（RT-qPCR）对进一步的 20 例脓毒症患者的脓毒症阶段和改善阶段进行了初步验证。最后，我们对 95 例脓毒症患者和 66 例非脓毒症患者进行了差异表达 miRNA 的验证。对验证的 miRNA 和患者的临床指标进行多元逻辑回归模型分析。通过分析接受者操作特征（ROC）曲线，确定并比较了变化 miRNA 在脓毒症中的诊断价值与 CRP 和 WBC。

结果

根据标准，我们检测到 miRNA 芯片调控的 11 个 miRNA。RT-qPCR 检测显示，脓毒症患者中 hsa-let-7d-3p 的表达较非脓毒症患者上调。在多变量逻辑回归分析中，血清 miRNA hsa-let-7d-3p 被发现是脓毒症的独立预测因子。ROC 分析显示，血清 hsa-let-7d-3p 的 ROC 曲线下面积为 0.696[95%置信区间（0.615，0.778）]。