Clinical Laboratory, The Fifth Hospital of Shanxi Medical University.
Department of Nephrology, The Fifth Hospital of Shanxi Medical University.
Tohoku J Exp Med. 2022 Mar;256(3):215-223. doi: 10.1620/tjem.256.215.
Immunoglobulin A nephropathy (IgAN) is the most common type of primary glomerulonephritis. It is very important to find new noninvasive biomarkers for the diagnosis and treatment of IgAN. The purpose of this study was to explore the clinical value of urinary exosomal miRNAs in IgAN. In this study, urinary exosomes were isolated from 29 IgAN patients and 29 healthy controls. The miRNA was analyzed by high-throughput sequencing. The expression of hsa-miR-451a and hsa-let-7d-3p was examined by real-time quantitative polymerase chain reaction (RT-qPCR). The diagnostic value of miRNAs was evaluated using receiver operating characteristic (ROC) curves. Here, hsa-miR-451a and hsa-let-7d-3p were upregulated in IgAN patients compared with healthy controls. We evaluated the diagnostic value of hsa-miR-451a and hsa-let-7d-3p using ROC curves; hsa-miR-451a (AUC = 0.805, p = 0.001), hsa-mir-7d-3p (AUC = 0.76, p = 0.0049), and the combination of hsa-miR-451a and hsa-let-7d-3p (AUC = 0.8125, p = 0.0007). Hsa-miR-451a has correlations with Lee's grades (r = 0.511, p = 0.021), and 24-h urinary protein excretion (UPE; r = 0.557, p = 0.011). Hsa-let-7d-3p showed correlations with Lee's grades (r = 0.6, p = 0.005), UPE (r = 0.518, p = 0.019), serum creatinine (r = 0.564, p = 0.01), and estimated glomerular filtration rate (r = -0.532, p = 0.016). According to the Oxford classification, for hsa-miR-451a, S0 had lower levels than S1 (p = 0.016); for hsa-mir-7d-3p, M0 had lower levels than M1 (p = 0.05). These findings suggest that hsa-miR-451a and hsa-let-7d-3p may serve as noninvasive biomarkers for the evaluation of IgAN.
免疫球蛋白 A 肾病（IgAN）是最常见的原发性肾小球肾炎。寻找新的非侵入性生物标志物对 IgAN 的诊断和治疗非常重要。本研究旨在探讨尿外泌体 miRNAs 在 IgAN 中的临床价值。在这项研究中，从 29 例 IgAN 患者和 29 例健康对照者中分离尿外泌体。通过高通量测序分析 miRNA。实时定量聚合酶链反应（RT-qPCR）检测 hsa-miR-451a 和 hsa-let-7d-3p 的表达。使用受试者工作特征（ROC）曲线评估 miRNA 的诊断价值。结果显示，与健康对照组相比，IgAN 患者的 hsa-miR-451a 和 hsa-let-7d-3p 表达上调。我们使用 ROC 曲线评估 hsa-miR-451a 和 hsa-let-7d-3p 的诊断价值；hsa-miR-451a（AUC=0.805，p=0.001），hsa-mir-7d-3p（AUC=0.76，p=0.0049），hsa-miR-451a 和 hsa-let-7d-3p 的组合（AUC=0.8125，p=0.0007）。hsa-miR-451a 与 Lee 分级（r=0.511，p=0.021）和 24 小时尿蛋白排泄量（UPE；r=0.557，p=0.011）相关。hsa-let-7d-3p 与 Lee 分级（r=0.6，p=0.005）、UPE（r=0.518，p=0.019）、血清肌酐（r=0.564，p=0.01）和估计肾小球滤过率（r=-0.532，p=0.016）相关。根据牛津分类，对于 hsa-miR-451a，S0 水平低于 S1（p=0.016）；对于 hsa-mir-7d-3p，M0 水平低于 M1（p=0.05）。这些发现表明 hsa-miR-451a 和 hsa-let-7d-3p 可能作为评估 IgAN 的非侵入性生物标志物。
