Department of Applied Science, William & Mary, 540 Landrum Drive, Williamsburg, Virginia, 23185, USA.
Department of Neuroscience, Jikei University School of Medicine, 3-25-8 Nishi-shimbashi, Minato, Tokyo, 105-8461, Japan.
Sci Data. 2022 Jul 30;9(1):457. doi: 10.1038/s41597-022-01569-y.
Neurons in the brainstem preBötzinger complex (preBötC) generate the rhythm and rudimentary motor pattern for inspiratory breathing movements. We performed whole-cell patch-clamp recordings from inspiratory neurons in the preBötC of neonatal mouse slices that retain breathing-related rhythmicity in vitro. We classified neurons based on their electrophysiological properties and genetic background, and then aspirated their cellular contents for single-cell RNA sequencing (scRNA-seq). This data set provides the raw nucleotide sequences (FASTQ files) and annotated files of nucleotide sequences mapped to the mouse genome (mm10 from Ensembl), which includes the fragment counts, gene lengths, and fragments per kilobase of transcript per million mapped reads (FPKM). These data reflect the transcriptomes of the neurons that generate the rhythm and pattern for inspiratory breathing movements.
脑桥preBötzinger 复合体（preBötC）中的神经元产生吸气呼吸运动的节律和基本运动模式。我们对保留体外呼吸节律性的新生小鼠脑片 preBötC 中的吸气神经元进行全细胞膜片钳记录。我们根据神经元的电生理特性和遗传背景对其进行分类，然后吸取其细胞内容物进行单细胞 RNA 测序（scRNA-seq）。该数据集提供了原始核苷酸序列（FASTQ 文件）和注释文件，这些文件将核苷酸序列映射到小鼠基因组（Ensembl 的 mm10），其中包括片段计数、基因长度以及每百万映射读取的每千碱基转录物的片段数（FPKM）。这些数据反映了产生吸气呼吸运动节律和模式的神经元的转录组。
