Zhengzhou Tobacco Research Institute of CNTC, Fengyang Street #2, Zhengzhou, Henan 450001, PR China.
Technology Center of China Tobacco Fujian Industrial Co., LTD, Binshui Road #298, Xiamen 361021, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2022 Oct 1;1208:123391. doi: 10.1016/j.jchromb.2022.123391. Epub 2022 Jul 26.
Heated tobacco products and electronic cigarettes are considered as alternatives to traditional tobacco cigarettes. However, it is crucial to monitor and compare the nicotine concentration in inhaled aerosols from these tobacco products, owing to the addictive nature and adverse effects of nicotine on human health. This study aimed to provide an electromembrane extraction (EME) combined liquid chromatography method to extract and determine nicotine in different inhaled aerosols. EME showed high extraction efficiency, selectivity, and sample clean-up capability. Under the optimal parameters, the linear range for nicotine was 0.1-200 mg L (r > 0.9998), and the limit of detection was 0.02 mg L. Good precision was obtained with the intra- and inter-day relative standard deviations of 2.2 % and 2.8 %, respectively. Repeatability was satisfactory (<7.7 %), and recoveries ranged from 81.0 % to 112.8 %. Finally, this method has been successfully used for the determination and comparison of nicotine in aerosols from these three tobacco products.
加热烟草制品和电子烟被认为是传统烟草卷烟的替代品。然而，由于尼古丁对人类健康的成瘾性和不良影响，监测和比较这些烟草制品吸入气溶胶中的尼古丁浓度至关重要。本研究旨在提供一种电迁移萃取（EME）结合液相色谱法，用于从不同吸入气溶胶中提取和测定尼古丁。EME 表现出高的萃取效率、选择性和样品净化能力。在最佳参数下，尼古丁的线性范围为 0.1-200mg/L(r>0.9998)，检出限为 0.02mg/L。日内和日间相对标准偏差分别为 2.2%和 2.8%，精密度良好。重复性令人满意（<7.7%），回收率在 81.0%至 112.8%之间。最后，该方法已成功用于这三种烟草制品气溶胶中尼古丁的测定和比较。
