Department of Child Healthcare, Luzhou people's Hospital, Luzhou, China.
Department of Pharmacy, Luzhou people's Hospital, Luzhou, China.
J Clin Lab Anal. 2022 Sep;36(9):e24620. doi: 10.1002/jcla.24620. Epub 2022 Jul 31.
We attempted to understand the status of vitamin (Vit) A, D, and E in children aged 0-6 living in the Tibetan plateau areas of Ganzi prefecture, to provide the basis for relevant government departments to carry out physical examinations of these children and to prevent and cure four key diseases (Infantile diarrhea, nutritional anemia, rickets, and infantile pneumonia).
Serum retinol and tocopherol levels were detected using high-performance liquid chromatography (HPLC). Serum levels of 25-(OH)D were detected by high-performance liquid chromatography-tandem mass spectrometry (LC-MS). The polynomial logistic regression was used to analyze the effects of age, season, altitude, and gender on Vit A, D, and E levels.
Vit A and D had the lowest mean serum levels before the age of 1 year and with the most significant deficiency rates. The lowest Vit E levels were seen in the Toddlerhood group. The rates of deficiency and insufficiency were the highest. Vit A, D, and E levels were significantly affected by seasonal changes and were significantly higher in the summer than in any other season. Vit A and D were significantly affected by altitude, and their levels were lowest above 4 km.
The overall levels of Vit A, D, and E in children aged 0-6 in the Tibetan plateau areas of Ganzi prefecture were lower than those in the plain's areas.
了解甘孜州藏区 0-6 岁儿童维生素（Vit）A、D、E 的现状，为相关政府部门开展儿童体检，防治婴幼儿腹泻、营养性贫血、佝偻病、婴幼儿肺炎等四类重点疾病提供依据。
采用高效液相色谱法（HPLC）检测血清视黄醇和生育酚水平，采用高效液相色谱-串联质谱法（LC-MS）检测血清 25-（OH）D 水平。采用多项式 logistic 回归分析年龄、季节、海拔和性别对 Vit A、D、E 水平的影响。
Vit A、D 在 1 岁前的平均血清水平最低，缺乏率最显著；Vit E 在幼儿期的血清水平最低，缺乏和不足率最高。Vit A、D 受季节变化的显著影响，夏季水平显著高于其他季节。Vit A、D 受海拔影响显著，海拔 4km 以上水平最低。
甘孜州藏区 0-6 岁儿童 Vit A、D、E 的总体水平低于平原地区。
