Tao Kin P, Chong Marc K C, Chan Kathy Y Y, Pun Jason C S, Tsun Joseph G S, Chow Samuel M W, Ng Calvin S H, Wang Maggie H T, Chan Paul K S, Li Albert M, Chan Renee W Y
Department of Paediatrics, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China.
CUHK-UMCU Joint Research Laboratory of Respiratory Virus & Immunobiology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China.
Curr Res Microb Sci. 2022 Jun 18;3:100147. doi: 10.1016/j.crmicr.2022.100147. eCollection 2022.
Influenza virus (IV) and the rhinovirus (RV) are the two most common circulating respiratory viruses circulating. Natural viral interference has been suggested between them. The effect of such at the population level has been described in temperate region, while its effect at the individual and cellular levels warrants further validation. In this study, we described the respiratory virus epidemiology and the co-infection landscape in the hospitalized population and investigated the distinct molecular pathways involved in the inhibition of virus replication.
Nasopharyngeal aspirates (NPAs) collected from patients during 2015 to 2019 were examined for the presence of respiratory viruses. The correlation of the monthly prevalence between all the tested respiratory viruses, the co-infection rate and the temporal interference of RV and IV were tested. The viral interference was validated by conducting sequential RV and IV infections in the well-differentiated primary human airway epithelial cells. The contributing molecular pathways were determined by transcriptome analysis.
A total of 112,926 NPAs were evaluated, and the Enterovirus/RV was the most prevalent respiratory virus detected. The negative correlation between EV/RV and IVs prevalence was independent of age and meteorological factors. Compare with other viruses, EV/RV had a significantly lower incidence of co-infection with IVs. Prior exposure to RV inhibited the replication of IV species A, B and oseltamivir-resistance stain . RV uniquely downregulated genes related to processing of viral mRNA, ribosomal proteins, translation and influenza infection.
Epidemiological surveillance and the sequential infection suggested viral interference between EV/RV and IV operates at the population, individual and cellular levels.
This study was supported by the General Research Fund (Ref: 24107017 and 14103119 to RWYC) and the Chinese University Direct Grant for Research (Ref: 2019·073 to RWYC).
流感病毒（IV）和鼻病毒（RV）是两种最常见的循环呼吸道病毒。有人提出它们之间存在天然病毒干扰。这种干扰在温带地区的人群水平上的影响已有描述，但其在个体和细胞水平上的影响仍需进一步验证。在本研究中，我们描述了住院人群中的呼吸道病毒流行病学和合并感染情况，并研究了参与抑制病毒复制的不同分子途径。
对2015年至2019年期间从患者采集的鼻咽抽吸物（NPA）进行呼吸道病毒检测。测试了所有检测的呼吸道病毒之间的月度流行率、合并感染率以及RV和IV的时间干扰之间的相关性。通过在分化良好的原代人呼吸道上皮细胞中进行连续的RV和IV感染来验证病毒干扰。通过转录组分析确定相关的分子途径。
共评估了112,926份NPA，肠道病毒/RV是检测到的最常见呼吸道病毒。EV/RV与IVs流行率之间的负相关与年龄和气象因素无关。与其他病毒相比，EV/RV与IVs的合并感染发生率显著较低。先前接触RV可抑制甲型、乙型流感病毒和耐奥司他韦毒株的复制。RV独特地下调了与病毒mRNA加工、核糖体蛋白、翻译和流感感染相关的基因。
流行病学监测和连续感染表明，EV/RV和IV之间的病毒干扰在人群、个体和细胞水平上起作用。
本研究得到了一般研究基金（编号：24107017和14103119，资助RWYC）和香港中文大学直接研究资助（编号：2019·073，资助RWYC）。
