Mektrirat Raktham, Rueangsri Thareerat, Keeratichandacha Waraporn, Soonsawat Sasiwimon, Boonyapakorn Chavalit, Pongkan Wanpitak
Department of Veterinary Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand.
Integrative Research Center for Veterinary Circulatory Sciences, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand.
Front Vet Sci. 2022 Jul 14;9:880952. doi: 10.3389/fvets.2022.880952. eCollection 2022.
Canine tracheal collapse is a progressive disease in small breed dogs resulting from chronic inflammation of the tracheal mucosal lining. Polyunsaturated fatty acid EAB-277 is one of the nutraceuticals that can alleviate inflammation and oxidative stress. Heart rate variability (HRV) is a prognostic tool related to sympathovagal balance and oxidative stress level, which is widely used with cardiorespiratory diseases. However, the effect of EAB-277 on HRV in tracheal collapse dogs has rarely been investigated. In this study, 26 tracheal collapse dogs were divided into two groups. In the control group, the dogs received the standard treatment, whereas the dogs in the EAB-277 group received standard treatment combined with EAB-277. After being treated for 5 weeks, changes in radiographic findings, blood profiles, serum malondialdehyde, inflammatory markers, and HRV were evaluated. This study found that clinical signs were improved in both groups ( < 0.05). However, serum malondialdehyde (MDA), Interleukin-6 (IL-6), and Tumor necrosis factor-alpha (TNF-α) were decreased only in the EAB-277 group after treatment for five weeks ( < 0.05) and the mean percent change of MDA, IL-6, and TNF-α at week five compared to baseline in the EAB-277 group was greater than in the control group ( < 0.05). Additionally, greater sympathovagal imbalance indicated by decreased standard deviation of all normal R-R intervals (SDNN) and standard deviation of the averaged R-R intervals for all 5-minutes segments (SDANN) was found in the control group at week five compared to baseline ( < 0.05), whereas EAB-277 improved SDNN and SDANN and decreased low frequency/high-frequency component (LF/HF ratio) after being treated for five weeks ( < 0.05). This study demonstrates that EAB-277 improves clinical signs and attenuates HRV impairment by reducing oxidative stress and inflammation in tracheal collapse dogs.
犬气管塌陷是一种小型犬类的进行性疾病，由气管黏膜内衬的慢性炎症引起。多不饱和脂肪酸EAB - 277是一种可减轻炎症和氧化应激的营养保健品。心率变异性（HRV）是一种与交感迷走神经平衡和氧化应激水平相关的预后工具，广泛应用于心肺疾病。然而，EAB - 277对气管塌陷犬HRV的影响鲜有研究。在本研究中，26只气管塌陷犬被分为两组。对照组的犬接受标准治疗，而EAB - 277组的犬接受标准治疗并联合EAB - 277。治疗5周后，评估影像学表现、血液指标、血清丙二醛、炎症标志物和HRV的变化。本研究发现两组的临床症状均有改善（<0.05）。然而，仅在EAB - 277组治疗5周后，血清丙二醛（MDA）、白细胞介素 - 6（IL - 6）和肿瘤坏死因子 - α（TNF - α）有所下降（<0.05），且EAB - 277组第5周时MDA、IL - 6和TNF - α相较于基线的平均变化百分比大于对照组（<0.05）。此外，与基线相比，对照组在第5周时所有正常R - R间期标准差（SDNN）和所有5分钟节段平均R - R间期标准差（SDANN）降低，提示交感迷走神经失衡更严重（<0.05），而EAB - 277治疗5周后改善了SDNN和SDANN，并降低了低频/高频成分（LF/HF比值）（<0.05）。本研究表明，EAB - 277可改善气管塌陷犬的临床症状，并通过减轻氧化应激和炎症来减轻HRV损伤。
