Li Ningning, Zhang Yongfeng, Wang Xuefeng, Ma Huailong, Sun Yaqing, Li Guolong, Zhang Shaoying
Sugar Beet Physiological Research Institute, Inner Mongolia Agricultural University, Hohhot, China.
Front Plant Sci. 2022 Jul 13;13:882753. doi: 10.3389/fpls.2022.882753. eCollection 2022.
Sugar beet taproot growth and development is a complex biological process involving morphogenesis and dry matter accumulation. However, the molecular regulatory mechanisms underlying taproot growth and development remain elusive. We performed a correlation analysis of the proteome and transcriptome in two cultivars (SD13829 and BS02) at the start and the highest points of the taproot growth rate. The corresponding correlation coefficients were 0.6189, 0.7714, 0.6803, and 0.7056 in four comparison groups. A total of 621 genes were regulated at both transcriptional and translational levels, including 190, 71, 140, and 220 in the BS59-VS-BS82, BS59-VS-SD59, BS82-VS-SD82, and SD59-VS-SD82 groups, respectively. Ten, 32, and 68 correlated-DEGs-DEPs (cor-DEGs-DEPs) were significantly enrdiched in the proteome and transcriptome of the BS59-VS-BS82, SD59-VS-SD82, and BS82-VS-SD82 groups, respectively, which included ribonuclease 1-like protein, DEAD-box ATP-dependent RNA helicase, TolB protein, heat shock protein 83, 20 kDa chaperonin, polygalacturonase, endochitinase, brassinolide and gibberellin receptors (BRI1 and GID1), and xyloglucan endotransglucosylase/hydrolase (XTH). In addition, XTH could enhance the growth and development of Arabidopsis primary roots by improving cell growth in the root tip elongation zone. These findings suggested that taproot growth and expansion might be regulated at transcriptional and posttranscriptional levels and also may be attributed to cell wall metabolism to improve cell wall loosening and elongation.
甜菜主根的生长发育是一个涉及形态发生和干物质积累的复杂生物学过程。然而，主根生长发育的分子调控机制仍不清楚。我们在两个品种（SD13829和BS02）主根生长速率的起始点和最高点进行了蛋白质组和转录组的相关性分析。四个比较组的相应相关系数分别为0.6189、0.7714、0.6803和0.7056。共有621个基因在转录和翻译水平上受到调控，其中在BS59-VS-BS82、BS59-VS-SD59、BS82-VS-SD82和SD59-VS-SD82组中分别有190、71、140和220个。在BS59-VS-BS82、SD59-VS-SD82和BS82-VS-SD82组的蛋白质组和转录组中，分别有10、32和68个相关差异基因-差异蛋白（cor-DEGs-DEPs）显著富集，其中包括核糖核酸酶1样蛋白、依赖ATP的DEAD盒RNA解旋酶、TolB蛋白、热休克蛋白83、20 kDa伴侣蛋白、多聚半乳糖醛酸酶、内切几丁质酶、油菜素内酯和赤霉素受体（BRI1和GID1）以及木葡聚糖内转糖基酶/水解酶（XTH）。此外，XTH可以通过改善根尖伸长区的细胞生长来促进拟南芥初生根的生长发育。这些发现表明，主根的生长和扩展可能在转录和转录后水平上受到调控，也可能归因于细胞壁代谢以促进细胞壁的松弛和伸长。
