Yao Qiushuang, Zhang Ze, Lv Xin, Chen Xiangyu, Ma Lulu, Sun Cong
The Key Laboratory of Oasis Eco-Agriculture, College of Agriculture, Shihezi University, Shihezi, China.
Front Plant Sci. 2022 Jul 13;13:920532. doi: 10.3389/fpls.2022.920532. eCollection 2022.
Potassium (K) is one of the most important elements influencing cotton metabolism, quality, and yield. Due to the characteristics of strong fluidity and fast redistribution of the K in plants, it leads to rapid transformation of the K lack or abundance in plant leaves; therefore, rapid and accurate estimation of potassium content in leaves (LKC, %) is a necessary prerequisite to solve the regulation of plant potassium. In this study, we concentrated on the LKC of cotton in different growth stages, an estimation model based on the combined characteristics of wavelet decomposition spectra and image was proposed, and discussed the potential of different combined features in accurate estimation of the LKC. We collected hyperspectral imaging data of 60 main-stem leaves at the budding, flowering, and boll setting stages of cotton, respectively. The original spectrum (R) is decomposed by continuous wavelet transform (CWT). The competitive adaptive reweighted sampling (CARS) and random frog (RF) algorithms combined with partial least squares regression (PLSR) model were used to determine the optimal decomposition scale and characteristic wavelengths at three growth stages. Based on the best "CWT spectra" model, the grayscale image databases were constructed, and the image features were extracted by using color moment and gray level co-occurrence matrix (GLCM). The results showed that the best decomposition scales of the three growth stages were CWT-1, 3, and 9. The best growth stage for estimating LKC in cotton was the boll setting stage, with the feature combination of "CWT-9 spectra + texture," and its determination coefficients ( val) and root mean squared error (RMSEval) values were 0.90 and 0.20. Compared with the single R model ( val = 0.66, RMSEval = 0.34), the val increased by 0.24. Different from our hypothesis, the combined feature based on "CWT spectra + color + texture" cannot significantly improve the estimation accuracy of the model, it means that the performance of the estimation model established with more feature information is not correspondingly better. Moreover, the texture features contributed more to the improvement of model performance than color features did. These results provide a reference for rapid and non-destructive monitoring of the LKC in cotton.
钾(K)是影响棉花新陈代谢、品质和产量的最重要元素之一。由于钾在植物体内流动性强且重新分配迅速,导致植物叶片中钾的缺乏或充足状态快速转变;因此,快速准确地估算叶片钾含量(LKC,%)是解决植物钾素调控问题的必要前提。在本研究中,我们聚焦于棉花不同生长阶段的LKC,提出了一种基于小波分解光谱和图像组合特征的估算模型,并探讨了不同组合特征在准确估算LKC方面的潜力。我们分别采集了棉花现蕾期、开花期和结铃期60片主茎叶的高光谱图像数据。原始光谱(R)通过连续小波变换(CWT)进行分解。采用竞争性自适应重加权采样(CARS)和随机蛙跳(RF)算法结合偏最小二乘回归(PLSR)模型来确定三个生长阶段的最佳分解尺度和特征波长。基于最佳的“CWT光谱”模型构建灰度图像数据库,并利用颜色矩和灰度共生矩阵(GLCM)提取图像特征。结果表明,三个生长阶段的最佳分解尺度分别为CWT - 1、3和9。棉花LKC估算的最佳生长阶段是结铃期,其特征组合为“CWT - 9光谱 + 纹理”,其决定系数(val)和均方根误差(RMSEval)值分别为0.90和0.20。与单一的R模型(val = 0.66,RMSEval = 0.34)相比,val提高了0.24。与我们的假设不同,基于“CWT光谱 + 颜色 + 纹理”的组合特征并不能显著提高模型的估算精度,这意味着利用更多特征信息建立的估算模型性能并非相应地更好。此外,纹理特征对模型性能提升的贡献比颜色特征更大。这些结果为棉花LKC的快速无损监测提供了参考。