Gong Zhenhua, Zhang Yi, Jiang Yasu, Chen Peng, Ji Jianfeng
Department of Burn and Plastic Surgery, Affiliated Hospital 2 of Nantong University, The First People's Hospital of Nantong, Nantong, China.
Nantong Clinical Medical College, Kangda College of Nanjing Medical University, Nantong, China.
J Oncol. 2022 Jul 22;2022:8145129. doi: 10.1155/2022/8145129. eCollection 2022.
This study investigated whether lncRNA NEAT1 could inhibit the proliferation of cutaneous squamous cell carcinoma (CSCC) cells by targeting miR-342-3p/CUL4B, thereby affecting the occurrence and development of CSCC.
Fluorescence quantitative PCR was used to detect the expression of lncRNA NEAT1 and miR-42-3p in skin squamous cell carcinoma and adjacent tissues. Bioinformatics software and luciferase reporter gene assay were used to analyze the association of lncRNA NEAT1 and miR-342-3p. The effect of overexpression or knockdown of miR-342-3p on the proliferation of CSCC cells was examined by MTT and colony formation assays. Western blotting was used to detect the proteins of the miR-342-3p/CUL4B signaling axis.
The lncRNA NEAT1 is abnormally overexpressed in CSCC tissues and cell lines. The expression of lncRNA NEAT1 and miR-342-3p in CSCC was negatively correlated. Bioinformatics prediction analysis revealed that lncRNA NEAT1 regulates the expression of miR-342-3p. The results of MTT and plate colony formation experiments showed that the transfection of miR-342-3p mimics significantly inhibited the proliferation and plate colony formation of CSCC cells, while the transfection of miR-342-3p inhibitor significantly promoted the proliferation and plate colony-forming ability of CSCC cells. Western blot results showed that lncRNA NEAT1 affected CSCC cell proliferation through miR-342-3p/CUL4B/PI3K-Akt signaling pathway.
The expression of lncRNA NEAT1 and miR-342-3p in CSCC tissues was negatively correlated. This study is the first to demonstrate that the lncRNA NEAT1, as a ceRNA, affects the proliferation of skin squamous cell carcinoma cells through the miR-342-3p/CUL4B/PI3K-Akt signaling pathway. Therefore, lncRNA NEAT1 could be a biological marker or target for CSCC diagnosis or treatment.
本研究旨在探究长链非编码RNA（lncRNA）NEAT1是否可通过靶向miR-342-3p/CUL4B抑制皮肤鳞状细胞癌（CSCC）细胞的增殖，从而影响CSCC的发生发展。
采用荧光定量PCR检测皮肤鳞状细胞癌组织及癌旁组织中lncRNA NEAT1和miR-342-3p的表达。运用生物信息学软件和荧光素酶报告基因检测分析lncRNA NEAT1与miR-342-3p的相关性。通过MTT法和集落形成实验检测过表达或敲低miR-342-3p对CSCC细胞增殖的影响。采用蛋白质免疫印迹法检测miR-342-3p/CUL4B信号轴相关蛋白。
lncRNA NEAT1在CSCC组织和细胞系中异常高表达。CSCC中lncRNA NEAT1与miR-342-3p的表达呈负相关。生物信息学预测分析显示lncRNA NEAT1可调控miR-342-3p的表达。MTT法和平板集落形成实验结果表明，转染miR-342-3p模拟物可显著抑制CSCC细胞的增殖及平板集落形成，而转染miR-342-3p抑制剂则显著促进CSCC细胞的增殖及平板集落形成能力。蛋白质免疫印迹结果表明，lncRNA NEAT1通过miR-342-3p/CUL4B/PI3K-Akt信号通路影响CSCC细胞增殖。
CSCC组织中lncRNA NEAT1与miR-342-3p的表达呈负相关。本研究首次证明lncRNA NEAT1作为竞争性内源RNA（ceRNA），通过miR-342-3p/CUL4B/PI3K-Akt信号通路影响皮肤鳞状细胞癌细胞的增殖。因此，lncRNA NEAT1可能成为CSCC诊断或治疗的生物学标志物或靶点。
