Drzaic Ivana, Curik Ino, Lukic Boris, Shihabi Mario, Li Meng-Hua, Kantanen Juha, Mastrangelo Salvatore, Ciani Elena, Lenstra Johannes A, Cubric-Curik Vlatka
Department of Animal Science, University of Zagreb Faculty of Agriculture, Zagreb, Croatia.
Department of Animal Production and Biotechnology, Faculty of Agrobiotechnical Sciences Osijek, Chair for Domestic Animal Breeding and Genetics, J. J. Strossmayer University of Osijek, Osijek, Croatia.
Front Genet. 2022 Jul 15;13:940736. doi: 10.3389/fgene.2022.940736. eCollection 2022.
A recent comprehensive genomic analysis based on 50K SNP profiles has shown that the regional Balkan sheep populations have considerable genetic overlap but are distinctly different from surrounding breeds. All eight Croatian sheep breeds were represented by a small number of individuals per breed. Here, we genotyped 220 individuals representing the native Croatian sheep breeds (Istrian Sheep, Krk Island Sheep, Cres Island Sheep, Rab Island Sheep, Lika Pramenka, Pag Island Sheep, Dalmatian Pramenka, Dubrovnik Sheep) and mouflon using the Ovine Infinium HD SNP BeadChip (606,006 SNPs). In addition, we included publicly available Balkan Pramenka and other Mediterranean sheep breeds. Our analyses revealed the complex population structure of Croatian sheep breeds and their origin and geographic barriers (island versus mainland). Migration patterns confirmed the historical establishment of breeds and the pathways of gene flow. Inbreeding coefficients (F) between sheep populations ranged from 0.025 to 0.070, with lower inbreeding coefficients observed in Dalmatian Pramenka and Pag Island Sheep and higher inbreeding in Dubrovnik sheep. The estimated effective population size ranged from 61 to 1039 for Krk Island Sheep and Dalmatian Pramenka, respectively. Higher inbreeding levels and lower effective population size indicate the need for improved conservation management to maintain genetic diversity in some breeds. Our results will contribute to breeding and conservation strategies of native Croatian sheep breeds.
最近一项基于50K SNP图谱的全面基因组分析表明，巴尔干地区的绵羊种群存在相当大的遗传重叠，但与周边品种明显不同。克罗地亚的所有八个绵羊品种每个品种的个体数量都很少。在此，我们使用绵羊Infinium HD SNP芯片（606,006个SNP）对代表克罗地亚本土绵羊品种（伊斯特拉绵羊、克尔克岛绵羊、 Cres岛绵羊、拉布岛绵羊、利卡普拉门卡羊、帕格岛绵羊、达尔马提亚普拉门卡羊、杜布罗夫尼克绵羊）和摩弗伦羊的220个个体进行了基因分型。此外，我们纳入了公开可用的巴尔干普拉门卡羊和其他地中海绵羊品种。我们的分析揭示了克罗地亚绵羊品种复杂的种群结构及其起源和地理障碍（岛屿与大陆）。迁移模式证实了品种的历史形成以及基因流动的途径。绵羊种群之间的近亲繁殖系数（F）在0.025至0.070之间，达尔马提亚普拉门卡羊和帕格岛绵羊的近亲繁殖系数较低，而杜布罗夫尼克绵羊的近亲繁殖系数较高。克尔克岛绵羊和达尔马提亚普拉门卡羊的估计有效种群大小分别为61至1039。较高的近亲繁殖水平和较低的有效种群大小表明需要改进保护管理以维持某些品种的遗传多样性。我们的结果将有助于克罗地亚本土绵羊品种的育种和保护策略。
