Vu Thi Thuy Hang, Kilian Adrzeij, Carling Jason, Lawn Robert John
College of Science and Engineering, James Cook University, Townsville, Qld 4811 Australia.
Faculty of Agronomy, Vietnam National University of Agriculture, Hanoi, Vietnam.
Physiol Mol Biol Plants. 2022 Jun;28(6):1277-1295. doi: 10.1007/s12298-022-01201-8. Epub 2022 Jul 1.
Mungbean is an important but understudied food legume compared with other major grain crops. Genetic studies through development of high-through put markers, linkage map construction and QTL analysis can accelerate and improve the efficiency of mining for genes for breeding in this crop. This study used four mungbean F recombinant inbred lines (RILs) from crosses of two wild types (ACC 1, ACC 87) and two cultivars (Berken, Kiloga) and DArT markers to construct individual and consensus linkage maps and to identify QTLs associated with 54 traits in mungbean. The number of polymorphic DArT markers identified among the four RIL populations varied from 1062 to 2013. The individual maps covered the lengths of 629.7-883.5 cM, comprising 672-981 DArT markers and 15-19 linkage groups (LG) with average distance between markers of 0.9-1.2 cM. The consensus map had the total length of 795.3 cM, comprising 1539 DArT markers and resolved 11 LGs with an average inter-marker distance of 0.65 cM. Sixty-two QTLs were identified for 39 traits across 10 LGs of the consensus map. Major QTLs were identified for two special traits, late flowering inherited from ACC 1 (6 QTLs, PVE of 11.2-29.9%) and perenniality inherited from ACC 87 (3 QTLs, PVE of 17.4-22.6%) in separate population analysis. Number of congruent QTLs across four mungbean populations and the consensus map was 18 for 13 traits. These results illustrated the high efficiency of DArT marker application in mungbean genetic dissection and suggested the future potential employment of identified QTLs for mungbean improvement.
与其他主要谷类作物相比，绿豆是一种重要但研究较少的食用豆类。通过开发高通量标记、构建连锁图谱和进行QTL分析的遗传研究，可以加速并提高该作物育种基因挖掘的效率。本研究使用了来自两个野生型（ACC 1、ACC 87）和两个栽培品种（Berken、Kiloga）杂交的四个绿豆F重组自交系（RIL）以及DArT标记，构建个体和整合连锁图谱，并鉴定与绿豆54个性状相关的QTL。在四个RIL群体中鉴定出的多态性DArT标记数量在1062至2013之间。个体图谱覆盖长度为629.7 - 883.5 cM，包含672 - 981个DArT标记和15 - 19个连锁群（LG），标记间平均距离为0.9 - 1.2 cM。整合图谱总长度为795.3 cM，包含1539个DArT标记，解析为11个LG，标记间平均距离为0.65 cM。在整合图谱的10个LG上，针对39个性状鉴定出62个QTL。在单独的群体分析中，针对两个特殊性状鉴定出主要QTL，即从ACC 1遗传的晚花（6个QTL，表型变异解释率为11.2 - 29.9%）和从ACC 87遗传的多年生性（3个QTL，表型变异解释率为17.4 - 22.6%）。四个绿豆群体和整合图谱上共有18个QTL与13个性状一致。这些结果表明DArT标记在绿豆遗传剖析中应用效率高，并暗示了未来利用已鉴定的QTL改良绿豆的潜力。
