Department of Plant Breeding and Genetics, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai, Tamil Nadu, India.
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK Gatersleben), Corrensstrasse 3, 06466, Seeland, Germany.
J Appl Genet. 2019 Nov;60(3-4):255-268. doi: 10.1007/s13353-019-00506-x. Epub 2019 Jul 22.
Mungbean (Vigna radiata) and ricebean (V. umbellata) were utilized to obtain an inter-specific recombinant inbred line (RIL) population with the objective of detecting quantitative trait loci (QTL) associated with mungbean yellow mosaic virus (MYMV) resistance. To precisely map QTLs, accurate genetic linkage maps are essential. In the present study, genotyping-by-sequencing (GBS) platform was utilized to develop the genetic linkage map. The map contained 538 single nucleotide polymorphism (SNP) markers, consisted of 11 linkage groups and spanned for 1291.7 cM with an average marker distance of 2.40 cM. The individual linkage group ranged from 90.2 to 149.1 cM in length, and the SNP markers were evenly distributed in the genetic linkage map, with 30-79 SNP markers per chromosome. The QTL analysis using the genetic map and 2 years (2015 and 2016) of phenotyping data identified five QTLs with phenotypic variation explained (PVE) from 10.11 to 20.04%. Of these, a QTL on chromosome 4, designated as qMYMV4-1, was major and stably detected in the same marker interval in both years. This QTL region harbours possible candidate genes for controlling MYMV resistance. The linkage map and QTL/gene (s) for MYMV resistance identified in this study should be useful for QTL fine mapping and cloning for further studies.
绿豆（Vigna radiata）和饭豆（V. umbellata）被用来获得一个种间重组近交系（RIL）群体，目的是检测与绿豆黄花叶病毒（MYMV）抗性相关的数量性状位点（QTL）。为了精确定位 QTL，准确的遗传连锁图谱是必不可少的。在本研究中，利用测序（GBS）平台开发了遗传连锁图谱。图谱包含 538 个单核苷酸多态性（SNP）标记，由 11 个连锁群组成，跨越 1291.7 cM，平均标记距离为 2.40 cM。每个连锁群的长度从 90.2 到 149.1 cM 不等，SNP 标记在遗传连锁图谱中均匀分布，每个染色体有 30-79 个 SNP 标记。利用遗传图谱和 2 年（2015 年和 2016 年）的表型数据进行 QTL 分析，鉴定出 5 个具有 10.11%至 20.04%表型变异解释（PVE）的 QTL。其中，第 4 号染色体上的一个 QTL，命名为 qMYMV4-1，在两年的相同标记区间中都是主要和稳定的。该 QTL 区域可能包含控制 MYMV 抗性的候选基因。本研究中鉴定的 MYMV 抗性的连锁图谱和 QTL/gene(s) 应该对 QTL 的精细定位和克隆研究有用。
