Schurkman Jacob, Liu Rui, Alavi Salma, Tandingan De Ley Irma, Hsiao Ansel, Dillman Adler R
Department of Nematology, University of California Riverside, Riverside, CA, United States.
Department of Microbiology and Plant Pathology, University of California Riverside, Riverside, CA, United States.
Front Microbiol. 2022 Jul 14;13:903136. doi: 10.3389/fmicb.2022.903136. eCollection 2022.
Nematodes in the genus can infect and kill slugs and snails, which are important agricultural pests. This useful trait has been commercialized by the corporation BASF after they mass produced a product labeled Nemaslug®. The product contains , which has been cultured with , a bacterial strain that was originally thought to be responsible for causing mortality in slugs and snails. The exact mechanism leading to death in a infected host is unknown but may involve contributions from nematode-associated bacteria. The naturally occurring microbial community of is unexplored; the previous microbial community studies have focused on laboratory grown or commercially reared nematodes, and in order to obtain a deeper understanding of the parasite and its host interactions, it is crucial to characterize the natural microbial communities associated with this organism in the wild. We sampled , , and directly from their habitats in Central and Southern California nurseries and garden centers and identified their native microbial community 16S amplicon sequencing. We found that the microbial community was influenced by species, location, and possibly gastropod host from which the nematode was collected. The predominant bacteria of the isolates collected included , , Aeromonadaceae, Pseudomonadaceae, and . isolates exhibited an enrichment with species belonging to Acinetobacter or Pseudomonadaceae. However, further research must be performed to determine if this is due to the location of isolate collection or a species specific microbial community pattern. More work on the natural microbial community of is needed to determine the role of bacteria in nematode virulence.
属的线虫可以感染并杀死蛞蝓和蜗牛，而它们是重要的农业害虫。巴斯夫公司在大规模生产了一种名为Nemaslug®的产品后，将这一有用特性商业化。该产品含有，它是与一种细菌菌株一起培养的，这种细菌菌株最初被认为是导致蛞蝓和蜗牛死亡的原因。导致受感染宿主死亡的确切机制尚不清楚，但可能涉及线虫相关细菌的作用。的自然微生物群落尚未得到探索；之前的微生物群落研究集中在实验室培养或商业饲养的线虫上，为了更深入地了解寄生虫及其与宿主的相互作用，表征与这种野生生物相关的自然微生物群落至关重要。我们直接从加利福尼亚州中部和南部苗圃及园艺中心的栖息地采集了、和，并通过16S扩增子测序鉴定了它们的天然微生物群落。我们发现的微生物群落受到物种、位置以及采集线虫的腹足类宿主的影响。所采集的分离株中的主要细菌包括、、气单胞菌科、假单胞菌科和。分离株显示出属于不动杆菌属或假单胞菌科的物种富集。然而，必须进行进一步研究以确定这是由于分离株采集的位置还是物种特异性的微生物群落模式。需要对的自然微生物群落开展更多研究，以确定细菌在线虫毒力中的作用。
