ZEB1可诱导人胶质母细胞瘤中N-钙黏蛋白的表达，并可能改变患者的生存期。
ZEB1 induces N-cadherin expression in human glioblastoma and may alter patient survival.
作者信息
Gött Hanna, Nagl Jasmin, Hagedorn Frederike, Thomas Samuel, Schwarm Frank P, Uhl Eberhard, Kolodziej Malgorzata A
机构信息
Department of Neurosurgery, University Hospital Giessen, D-35392 Giessen, Germany.
Department of Pathology, University Hospital Giessen, D-35392 Giessen, Germany.
出版信息
Mol Clin Oncol. 2022 Jun 8;17(2):123. doi: 10.3892/mco.2022.2556. eCollection 2022 Aug.
The present study investigated the expression of epithelial-mesenchymal transition (EMT)-related factors zinc finger E-box-binding homeobox 1 (ZEB1), cadherin-1 (CDH1), cadherin-2 (CDH2) and the cell cycle modulating kinase cyclin-dependent kinase 1 (CDK1) in human glioblastoma (GBM) compared to normal brain tissue, as well as whether the levels of expression were associated with the overall and progression-free survival of the GBM patients. In 44 GBM and five normal brain tissue specimens, the expression levels of ZEB1, CDH1, CDH2 and CDK1 were evaluated by real-time PCR and immunostaining, and the results were correlated with clinical data. The expression levels of all investigated genes as detected by immunostaining were significantly higher in the GBM when compared to the normal brain tissues. There was no influence on survival. A linear correlation between ZEB1 and CDH2 and CDK1 expression was observed in GBM. Moreover, ZEB1 was involved in EMT (e.g., signaling in human GBM) and high ZEB1 levels were linked to an aberrant cell cycle processing, marked by CDK1 overexpression.
本研究调查了与上皮-间质转化（EMT）相关的因子锌指E盒结合同源框1（ZEB1）、钙黏蛋白-1（CDH1）、钙黏蛋白-2（CDH2）以及细胞周期调节激酶细胞周期蛋白依赖性激酶1（CDK1）在人胶质母细胞瘤（GBM）中的表达，并与正常脑组织进行比较，同时探究这些因子的表达水平是否与GBM患者的总生存期和无进展生存期相关。在44例GBM和5例正常脑组织标本中，通过实时聚合酶链反应（PCR）和免疫染色评估ZEB1、CDH1、CDH2和CDK1的表达水平，并将结果与临床数据相关联。与正常脑组织相比，免疫染色检测到的所有研究基因在GBM中的表达水平均显著更高。但对生存期无影响。在GBM中观察到ZEB1与CDH2和CDK1表达之间存在线性相关性。此外，ZEB1参与EMT（如在人GBM中的信号传导），高ZEB1水平与异常的细胞周期进程相关，其特征为CDK1过表达。
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