Department of Orthopedics, Yangzhou Hongquan Hospital, Yangzhou, Jiangsu Province 225000, China.
Comput Math Methods Med. 2022 Jul 20;2022:2025756. doi: 10.1155/2022/2025756. eCollection 2022.
Spinal cord injury (SCI) is one of the most devastating central injuries, resulting in serious locomotor deficits. Triad1 is known to play an important role in SCI, but its effects on the inflammatory response and physiological behavior have not been thoroughly studied. This study is aimed at examining the effects of Triad1 on the inflammatory response and neuronal injury in acute SCI in rats.
Twenty-four male Sprague-Dawley (SD) rats were randomly divided into a control group, SCI group, sh-NC group, and Triad1 knockout group (sh-Triad1). The Basso Beattie Bresnahan locomotor rating scale was utilized for the assessment of the motor ability of rats. Hematoxylin and eosin (H&E), Luxol fast blue (LFB), and TUNEL staining were used to assess the pathological injury, demyelination, and neuronal apoptosis, respectively. ELISA was used to detect the levels of IL-1, IL-10, and TNF-, and qRT-PCR was used to examine the expression level of Triad1. Furthermore, the protein levels of Triad1, Bax, Bcl-2, and cleaved caspase-3 were determined using western blotting.
The Triad1 expression level was upregulated in damaged spinal cord tissue. Knockdown of Triad1 improved motor function and reduced SCI as well as apoptosis of spinal cord neurons. In addition, the knockdown of Triad1 inhibited the inflammatory response caused by SCI.
Knockdown of Triad1 can reduce SCI in rats with acute SCI by inhibiting the inflammatory response and apoptosis.
脊髓损伤（SCI）是最严重的中枢神经系统损伤之一，导致严重的运动功能障碍。Triad1 已知在 SCI 中发挥重要作用，但它对炎症反应和生理行为的影响尚未得到充分研究。本研究旨在研究 Triad1 对急性 SCI 大鼠炎症反应和神经元损伤的影响。
24 只雄性 Sprague-Dawley（SD）大鼠随机分为对照组、SCI 组、sh-NC 组和 Triad1 敲除组（sh-Triad1）。Basso Beattie Bresnahan 运动评分量表用于评估大鼠的运动能力。苏木精和伊红（H&E）、卢索快速蓝（LFB）和 TUNEL 染色分别用于评估病理损伤、脱髓鞘和神经元凋亡。ELISA 用于检测白细胞介素-1（IL-1）、白细胞介素-10（IL-10）和肿瘤坏死因子-α（TNF-α）的水平，qRT-PCR 用于检测 Triad1 的表达水平。此外，使用 Western blot 检测 Triad1、Bax、Bcl-2 和 cleaved caspase-3 的蛋白水平。
Triad1 表达水平在受损脊髓组织中上调。Triad1 敲低改善了运动功能，减轻了 SCI 大鼠的脊髓神经元凋亡。此外，Triad1 敲低抑制了 SCI 引起的炎症反应。
Triad1 敲低可通过抑制炎症反应和细胞凋亡来减轻急性 SCI 大鼠的 SCI。
