Zuo Fengli, Yu Jing, He Xiujing
Laboratory of Integrative Medicine, Clinical Research Center for Breast, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center, Chengdu, China.
Front Oncol. 2022 Jul 13;12:931393. doi: 10.3389/fonc.2022.931393. eCollection 2022.
Aberrant metabolism contributes to tumor initiation, progression, metastasis, and drug resistance. Metabolic dysregulation has emerged as a hallmark of several hematologic malignancies. Decoding the molecular mechanism underlying metabolic rewiring in hematological malignancies would provide promising avenues for novel therapeutic interventions. Single-cell metabolic analysis can directly offer a meaningful readout of the cellular phenotype, allowing us to comprehensively dissect cellular states and access biological information unobtainable from bulk analysis. In this review, we first highlight the unique metabolic properties of hematologic malignancies and underscore potential metabolic vulnerabilities. We then emphasize the emerging single-cell metabolomics techniques, aiming to provide a guide to interrogating metabolism at single-cell resolution. Furthermore, we summarize recent studies demonstrating the power of single-cell metabolomics to uncover the roles of metabolic rewiring in tumor biology, cellular heterogeneity, immunometabolism, and therapeutic resistance. Meanwhile, we describe a practical view of the potential applications of single-cell metabolomics in hematopoiesis and hematological malignancies. Finally, we present the challenges and perspectives of single-cell metabolomics development.
异常代谢有助于肿瘤的起始、进展、转移和耐药性。代谢失调已成为几种血液系统恶性肿瘤的一个标志。解码血液系统恶性肿瘤中代谢重编程的分子机制将为新型治疗干预提供有前景的途径。单细胞代谢分析可以直接提供有意义的细胞表型读数，使我们能够全面剖析细胞状态并获取从整体分析中无法获得的生物学信息。在本综述中，我们首先强调血液系统恶性肿瘤独特的代谢特性，并强调潜在的代谢脆弱性。然后，我们着重介绍新兴的单细胞代谢组学技术，旨在为以单细胞分辨率研究代谢提供指导。此外，我们总结了最近的研究，这些研究证明了单细胞代谢组学在揭示代谢重编程在肿瘤生物学、细胞异质性、免疫代谢和治疗耐药性中的作用方面的能力。同时，我们描述了单细胞代谢组学在造血和血液系统恶性肿瘤中潜在应用的实际观点。最后，我们提出了单细胞代谢组学发展面临的挑战和前景。
