Bratisl Lek Listy. 2022;123(10):724-729. doi: 10.4149/BLL_2022_116.
The aim of this study was to determine the susceptibility of Staphylococcus aureus strains to commercial phage preparations. The strains were isolated from clinical patients as well as from nasal mucosa of healthy carriers.
The elevating number of antibiotic-resistant S. aureus strains present a therapeutic challenge, especially in high-risk patients. One of the promising ways to solve this problem is phage therapy.
Susceptibility of 111 carrier strains of S. aureus (4 strains were methicillin-resistant; MRSA) and 81 clinical isolates from bloodstream or skin and soft tissue infections (28 were MRSA) to four commercial phage preparations was assessed in vitro by spot assay. The clonality of S. aureus strains was determined by spa typing.
Spa typing revealed 97 distinct spa types. A proportion of 73-80 % of the tested S. aureus strains were revealed to have in vitro phage susceptibility, depending on the clonal affiliation of the strains and phage preparation tested. The susceptibility to phage preparations was significantly higher in MRSA strains (p < 0.001).
In vitro results indicate a promising therapeutic potential of the tested commercial anti-staphylococcal phage preparations. They could be applied to a broad spectrum of bacterial clones, and have an excellent activity especially against MRSA strains (Tab. 2, Fig. 2, Ref. 43).
本研究旨在确定金黄色葡萄球菌（Staphylococcus aureus）菌株对商业噬菌体制剂的敏感性。这些菌株是从临床患者和健康携带者的鼻腔黏膜中分离出来的。
越来越多的抗生素耐药性金黄色葡萄球菌菌株带来了治疗挑战，尤其是对高风险患者。解决这个问题的一种有前途的方法是噬菌体疗法。
通过点试验评估了 111 株来自鼻腔携带菌（4 株为耐甲氧西林金黄色葡萄球菌；MRSA）和 81 株来自血流或皮肤和软组织感染的临床分离株（28 株为 MRSA）对四种商业噬菌体制剂的敏感性。金黄色葡萄球菌菌株的克隆性通过 spa 分型确定。
spa 分型显示 97 种不同的 spa 型。根据菌株的克隆亲缘关系和测试的噬菌体制剂，测试的金黄色葡萄球菌菌株中有 73-80%具有体外噬菌体敏感性。噬菌体制剂对 MRSA 菌株的敏感性显著更高（p<0.001）。
体外结果表明，测试的商业抗金黄色葡萄球菌噬菌体制剂具有有前途的治疗潜力。它们可以应用于广泛的细菌克隆，并且对 MRSA 菌株具有极好的活性（表 2，图 2，参考文献 43）。
