Zheng Binyu, Song Ningying, Wu Huizhen, Tong Jianying, Sun Nabo
College of Environment, Zhejiang University of Technology, Hangzhou 310032, China.
College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China.
Anal Methods. 2022 Aug 11;14(31):2976-2981. doi: 10.1039/d2ay00885h.
The residue of antibiotics in the soil is becoming more and more common, which may affect the normal growth of plants and organisms. The aim of this study was to investigate the residues of antibiotics in tea gardens' soil after a long-term application of manure. An ultra-high performance liquid chromatography-tandem mass spectrometry method was developed to simultaneously determine the residues of 32 antibiotics in the soil of tea gardens after fertilization. The samples were extracted with methanol-acetonitrile and purified with C18 at the same time. Then, mixed dispersive sorbents dispersed in a syringe were used for the second purification. The results showed that the antibiotics have a good linear relationship within the range. The recovery rate is 70.1-120.3%. The applicability of the method was demonstrated by analyzing 30 real samples (with a detection rate of 43.3%). The method is a simple and environmentally friendly method for the analysis of multiple antibiotics in soils, and it could provide a basis for the risk assessment of antibiotics in agricultural environments and the standard application of organic fertilizers in tea gardens.
土壤中抗生素残留越来越普遍，这可能影响植物和生物的正常生长。本研究旨在调查长期施用粪肥后茶园土壤中抗生素的残留情况。建立了一种超高效液相色谱-串联质谱法，用于同时测定施肥后茶园土壤中32种抗生素的残留量。样品用甲醇-乙腈提取，同时用C18净化。然后，使用分散在注射器中的混合分散吸附剂进行二次净化。结果表明，抗生素在该范围内具有良好的线性关系。回收率为70.1-120.3%。通过分析30个实际样品（检出率为43.3%）证明了该方法的适用性。该方法是一种简单且环保的土壤中多种抗生素分析方法，可为农业环境中抗生素的风险评估及茶园有机肥的规范施用提供依据。
