Twardowski J, Lityńska A, Proniewicz L M, Domosławski J
Chronobiologia. 1981 Jul-Sep;8(3):231-42.
The activity of acid phosphatase (E.C.3.1.3.2.), arylsulfatase (E.C.3.1.1.23.), beta-galactosidase (E.C.3.1.1.23.), and beta-acetylglucosaminidase (E.C.3.2.1.30.) in rat liver homogenates of 4.5 month-old male rats is presented in this paper. The degradation processes are observed in rat liver homogenate after incubation. The activity of acid phosphatase and beta-acetylglucosaminidase increases, the activity in one of beta-galactosidase is constant, and arylsulfatase decreases during the time of incubation. Furthermore, the maxima of the enzyme activities shift during the incubation in the time of a day. Gel filtration of acid phosphatase on the Sephadex G-150 Superfine and DEAE-cellulose columns determinate the mutual content of acid phosphatase subunits to isoenzymes I and II in various points of a day. The greatest content of acid phosphatase subunits versus both the isoenzymes content is at 02(24), and the greatest content of isoenzyme II versus the content of isoenzymes I appears at 07(12). From these data it is clear that the period of the isoenzyme II synthesis from the subunit amounts to 5 h, while 10 h are necessary to create the isoenzyme I originated from isoenzyme II. The comparison of acid phosphatase activity before and after the homogenate filtration on the Sephadex column indicates the increase of this enzyme activity after its separation from the other proteins and other components.
本文展示了4.5月龄雄性大鼠肝脏匀浆中酸性磷酸酶（E.C.3.1.3.2.）、芳基硫酸酯酶（E.C.3.1.1.23.）、β-半乳糖苷酶（E.C.3.1.1.23.）和β-乙酰氨基葡萄糖苷酶（E.C.3.2.1.30.）的活性。在孵育后观察大鼠肝脏匀浆中的降解过程。在孵育期间，酸性磷酸酶和β-乙酰氨基葡萄糖苷酶的活性增加，β-半乳糖苷酶中的一种活性保持恒定，而芳基硫酸酯酶的活性降低。此外，酶活性的最大值在一天中的孵育期间发生变化。在Sephadex G-150 Superfine和DEAE-纤维素柱上对酸性磷酸酶进行凝胶过滤，确定了一天中不同时间点酸性磷酸酶亚基与同工酶I和II的相互含量。相对于两种同工酶含量，酸性磷酸酶亚基的最大含量出现在02(24)，而异工酶II相对于同工酶I含量的最大含量出现在07(12)。从这些数据可以清楚地看出，从亚基合成同工酶II的时间为5小时，而从同工酶II产生同工酶I则需要10小时。在Sephadex柱上对匀浆进行过滤前后酸性磷酸酶活性的比较表明，该酶与其他蛋白质和其他成分分离后活性增加。
