Center for Viticulture and Enology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
Folia Microbiol (Praha). 2023 Feb;68(1):73-86. doi: 10.1007/s12223-022-00996-z. Epub 2022 Aug 1.
Bacillus species as fungal antagonistic agents have been widely used in the agriculture and considered as safe products for the management of plant pathogens. In this study, we reported the whole genome sequence of strain LJBV19 isolated from grapevine rhizosphere soil. Strain LJBV19 was identified as Bacillus velezensis through morphological, physicochemical, molecular analysis and genome comparison. Bacillus velezensis LJBV19 had a significant inhibitory effect on the growth of Magnaporthe oryzae with an inhibition ratio up to 75.55% and showed broad spectrum of activity against fungal phytopathogens. The 3,973,013-bp circular chromosome with an average GC content of 46.5% consisted of 3993 open reading frames (ORFs), and 3308 ORFs were classified into 19 cluster of orthologous groups of proteins (COG) categories. Genes related to cell wall degrading enzymes were predicted by Carbohydrate-Active enZYmes (CAZy) database and validated at the metabolic level, producing 0.53 ± 0.00 U/mL cellulose, 0.14 ± 0.01 U/mL chitinase, and 0.11 ± 0.01 U/mL chitosanase. Genome comparison confirmed the taxonomic position of LJBV19, conserved genomic structure, and genetic homogeneity. Moreover, 13 gene clusters for biosynthesis of secondary metabolites in LJBV19 genome were identified and two unique clusters (clusters 2 and 12) shown to direct an unknown compound were only present in strain LJBV19. In general, our results will provide insights into the antifungal mechanisms of Bacillus velezensis LJBV19 and further application of the strain.
芽孢杆菌作为真菌拮抗物在农业中得到了广泛应用，并被认为是管理植物病原体的安全产品。在本研究中，我们报道了从葡萄根际土壤中分离得到的菌株 LJBV19 的全基因组序列。通过形态学、理化、分子分析和基因组比较，菌株 LJBV19 被鉴定为贝莱斯芽孢杆菌。贝莱斯芽孢杆菌 LJBV19 对稻瘟病菌的生长具有显著的抑制作用，抑制率高达 75.55%，并对真菌植物病原体表现出广谱的活性。该 3973013bp 的环状染色体平均 GC 含量为 46.5%，包含 3993 个开放阅读框（ORF），其中 3308 个 ORF 分为 19 个同源基因簇（COG）类别。通过碳水化合物活性酶（CAZy）数据库预测与细胞壁降解酶相关的基因，并在代谢水平上进行验证，产生 0.53±0.00 U/mL 纤维素、0.14±0.01 U/mL 几丁质酶和 0.11±0.01 U/mL 壳聚糖酶。基因组比较证实了 LJBV19 的分类地位、保守的基因组结构和遗传同质性。此外，在 LJBV19 基因组中鉴定出 13 个用于次生代谢物合成的基因簇，其中两个独特的簇（簇 2 和 12）仅存在于菌株 LJBV19 中，表明其直接产生未知化合物。总的来说，我们的研究结果将为深入了解贝莱斯芽孢杆菌 LJBV19 的抗真菌机制以及该菌株的进一步应用提供依据。
