贝莱斯芽孢杆菌Lzh-5的全基因组序列及比较分析，该菌株具有真菌拮抗和促进植物生长的特性
Complete genome sequence and comparative analysis of Bacillus velezensis Lzh-5, a fungal antagonistic and plant growth-promoting strain.
作者信息
Zhao Dongying, Wu Yutong, Qu Jie, Fang Lei, Liu Chaoyue, Zhang Lin, Zhang Mingshuo, Wang Jihua, Li Zhenghua
机构信息
Shandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, 253023, P. R. China.
Shandong Engineering Laboratory of Swine Herd Health Big Data and Intelligent Monitoring, Dezhou University, Dezhou, 253023, P. R. China.
出版信息
BMC Microbiol. 2025 Apr 22;25(1):230. doi: 10.1186/s12866-025-03938-0.
BACKGROUND
Plant diseases significantly and persistently impair product quality and yield across the globe. Employing antagonistic microorganisms represents an environmentally friendly and cost-effective approach to pathogen management. In this study, Bacillus velezensis Lzh-5 was explored to understand the molecular underpinnings of its antagonistic activity and plant growth-promoting properties.
RESULTS
We present the basic genomic profile of B. velezensis Lzh-5. Whole-genome analysis revealed that Lzh-5 possesses a 4,015,817 bp circular chromosome with a GC content of 46.0%, and an 8,933 bp circular plasmid with a GC content of 40.5%. A total of 3,998 genes were predicted, of which 3,881 (97.07%) are coding DNA sequences (CDSs). Through phylogenomic and comparative genomic analyses, strain Lzh-5 was confirmed as B. velezensis. The Lzh-5 genome harbors genes for cell wall-degrading enzymes. Additionally, 13 gene clusters responsible for secondary metabolite production were identified. Notably, a unique cluster (cluster 2) coding for an unknown compound was found exclusively in strain Lzh-5. Genes associated with plant growth enhancement, such as those involved in chemotaxis, motility, biofilm formation, phytohormone production, nitrogen fixation, phosphate solubilization, glycine betaine biosynthesis, and acetoin and 2,3-butanediol synthesis, were also identified.
CONCLUSION
The basic characteristics of strain Lzh-5 genome were delineated through whole-genome sequencing. Our analysis indicates that the Lzh-5 genome encompasses various genes that promote plant growth, induce systemic resistance, and antagonize pathogens. Compared to other strains, several unique gene clusters in Lzh-5 may contribute to the discovery of novel bioactive compounds and offer a broader antagonistic spectrum. This investigation elucidates the antifungal and plant growth-promoting mechanisms of B. velezensis Lzh-5 at a genetic level, providing a theoretical foundation for further application in agricultural production.
背景
植物病害在全球范围内严重且持续地损害产品质量和产量。利用拮抗微生物是一种环境友好且经济高效的病原体管理方法。在本研究中，对贝莱斯芽孢杆菌Lzh-5进行了探索，以了解其拮抗活性和促进植物生长特性的分子基础。
结果
我们展示了贝莱斯芽孢杆菌Lzh-5的基本基因组概况。全基因组分析表明，Lzh-5拥有一条4,015,817 bp的环状染色体，GC含量为46.0%，以及一个8,933 bp的环状质粒，GC含量为40.5%。共预测到3,998个基因，其中3,881个（97.07%）为编码DNA序列（CDS）。通过系统发育基因组学和比较基因组学分析，菌株Lzh-5被确认为贝莱斯芽孢杆菌。Lzh-5基因组含有细胞壁降解酶的基因。此外，还鉴定出13个负责次生代谢产物产生的基因簇。值得注意的是，在菌株Lzh-5中发现了一个独特的编码未知化合物的基因簇（簇2）。还鉴定出了与促进植物生长相关的基因，如参与趋化性、运动性、生物膜形成、植物激素产生、固氮、磷溶解、甘氨酸甜菜碱生物合成以及3-羟基丁酮和2,3-丁二醇合成的基因。
结论
通过全基因组测序描绘了菌株Lzh-5基因组的基本特征。我们的分析表明，Lzh-5基因组包含多种促进植物生长、诱导系统抗性和拮抗病原体的基因。与其他菌株相比，Lzh-5中的几个独特基因簇可能有助于发现新型生物活性化合物并提供更广泛的拮抗谱。本研究在遗传水平上阐明了贝莱斯芽孢杆菌Lzh-5的抗真菌和促进植物生长机制，为其在农业生产中的进一步应用提供了理论基础。
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