Qi Yufeng, Wu Haodong, Liu Conghui, Zheng Danni, Yan Congzhi, Hu Wenjing, Zhang Xiaohua, Dai Xuanxuan
Department of Thyroid and Breast Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China.
J Breast Cancer. 2022 Aug;25(4):327-343. doi: 10.4048/jbc.2022.25.e26. Epub 2022 Jun 13.
The incidence rate of breast cancer (BC) has increased annually. Downstream neighbor of son (DONSON) critically affects cell cycle progression and maintains stable genomic properties; however, its relevant effects on BC growth and progression require in-depth investigation.
DONSON upregulation was validated in public databases. DONSON expression in matched BC and adjacent tissues and cell lines (MDA-MB-231, BT-549, and HS-578T) was determined using quantitative reverse transcription polymerase chain reaction. apoptosis, invasion, migration, and proliferation tests were performed to ascertain the functions of DONSON in BC cell lines. Then, using western blot analysis, the levels of DONSON downstream proteins were determined.
Compared to the control, DONSON was expressed at higher levels in BC tissues and cell lines. DONSON knockdown facilitated apoptosis and limited proliferation, migration, invasion, and S/G2 transition of BC cells . Furthermore, DONSON overexpression promoted BC cell proliferation and inhibited apoptosis . Moreover, DONSON knockdown reduced cyclin A1 and cyclin-dependent kinase 2 levels. Moreover, DONSON knockdown limited the progression of epithelial-mesenchymal transition.
DONSON critically affects BC growth and serves as a possible target and marker for the efficacy of subsequent therapies.
乳腺癌（BC）的发病率逐年上升。儿子的下游邻居（DONSON）对细胞周期进程有重要影响并维持基因组特性的稳定；然而，其对BC生长和进展的相关影响需要深入研究。
在公共数据库中验证了DONSON的上调。使用定量逆转录聚合酶链反应测定了DONSON在匹配的BC组织和相邻组织以及细胞系（MDA-MB-231、BT-549和HS-578T）中的表达。进行凋亡、侵袭、迁移和增殖试验以确定DONSON在BC细胞系中的功能。然后，使用蛋白质印迹分析测定DONSON下游蛋白的水平。
与对照组相比，DONSON在BC组织和细胞系中表达水平更高。敲低DONSON促进了BC细胞的凋亡并限制了其增殖、迁移、侵袭和S/G2期转换。此外，过表达DONSON促进了BC细胞的增殖并抑制了凋亡。此外，敲低DONSON降低了细胞周期蛋白A1和细胞周期蛋白依赖性激酶2的水平。而且，敲低DONSON限制了上皮-间质转化的进程。
DONSON对BC生长有重要影响，可作为后续治疗疗效的潜在靶点和标志物。
