Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Constituent College of Pakistan Institute of Engineering and Applied Sciences, Jhang Road, Faisalabad, Pakistan.
Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Constituent College of Pakistan Institute of Engineering and Applied Sciences, Jhang Road, Faisalabad, Pakistan.
Trends Biotechnol. 2022 Oct;40(10):1141-1143. doi: 10.1016/j.tibtech.2022.07.005. Epub 2022 Jul 29.
The CRISPR-Cas toolbox is expanding swiftly. Every discovery of a novel and unique variant opens new frontiers in the field of synthetic and applied biology. Recently, Huang et al. revealed the CRISPR-Cas12c system that requires a single-nucleotide protospacer adjacent motif (PAM) to perform RNA-guided DNA targeting without DNA cleavage.
CRISPR-Cas 工具包正在迅速扩展。每一项新的独特变体的发现都为合成和应用生物学领域开辟了新的前沿。最近，Huang 等人揭示了 CRISPR-Cas12c 系统，该系统需要单个核苷酸的原间隔序列邻近基序 (PAM) 才能在不进行 DNA 切割的情况下执行 RNA 引导的 DNA 靶向。
