Department of Anesthesiology, Jinling Hospital, Nanjing Medical University, Nanjing, P.R. China.
Department of Anesthesiology, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi, P.R. China.
J Int Med Res. 2022 Jul;50(8):3000605221115388. doi: 10.1177/03000605221115388.
To determine if the beneficial effects of transient desflurane application mitigates inflammation and decrease associated signaling induced by 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) in mice.
Mice were induced to develop Parkinson's disease (PD) by intraperitoneal injection with MPTP for 20 consecutive days, and validated mice were randomly allocated to four groups. Collected samples from euthanized mice were designated for the following analyses: 1) immunohistochemical staining for positive dopaminergic neurons in the substantia nigra and striatum, 2) immunofluorescence staining for ionized calcium binding adaptor molecule-1 (Iba1) and glial fibrillary acid protein (GFAP), and 3) western blotting for p38, p-p38, toll-like receptor 4, and tumor necrosis factor (TNF)-α.
The inhalation of desflurane for 1 hour ameliorated locomotory dysfunctions of PD mice by recovering the loss of Iba1- and GFAP-positive dopaminergic neurons, deactivating microglial cells and astrocytes, and decreasing the amounts of inflammatory cytokines (TNF-α).
These findings suggest that transient desflurane inhalation may provide some benefits for PD through ameliorating inflammation and enhancing locomotor activity.
确定短暂七氟醚应用的有益效果是否可以减轻炎症并降低 1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）诱导的小鼠相关信号转导。
通过腹腔注射 MPTP 连续 20 天诱导小鼠发生帕金森病（PD），并验证合格的小鼠随机分为四组。从安乐死的小鼠中收集样本，用于以下分析：1）黑质和纹状体中阳性多巴胺能神经元的免疫组织化学染色，2）离子钙结合衔接蛋白-1（Iba1）和胶质纤维酸性蛋白（GFAP）的免疫荧光染色，以及 3）p38、p-p38、Toll 样受体 4 和肿瘤坏死因子（TNF）-α的 Western 印迹。
七氟醚吸入 1 小时可改善 PD 小鼠的运动功能障碍，恢复 Iba1 和 GFAP 阳性多巴胺能神经元的丧失，使小胶质细胞和星形胶质细胞失活，并减少炎症细胞因子（TNF-α）的含量。
这些发现表明，短暂七氟醚吸入可能通过减轻炎症和增强运动活动为 PD 提供一些益处。
