Kang Taemin, Yim Donggyun, Baek Ki Ho, Lee Yee Eun, Kim Hyun-Jun, Jo Cheorun
Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul, Republic of Korea.
Department of Nano-Bio Convergence, Korea Institute of Materials Science, Changwon, Republic of Korea.
J Appl Microbiol. 2022 Nov;133(5):3007-3019. doi: 10.1111/jam.15757. Epub 2022 Aug 9.
This study aimed to examine the inactivation efficacy of plasma-activated acetic acid (PAAA) against Salmonella Typhimurium cells and biofilm and elucidate underlying chemical inactivation pathway.
PAAA was prepared by discharging plasma to 20 ml of 0.2% (v/v) acetic acid (AA) for 20 min (2.2 kHz and 8.4 kVpp). The count of cells and biofilms decreased by 5.71 log CFU ml and 4 log CFU/cm after 10 min of treatment with 0.2% PAAA and 0.4% PAAA compared with control group (without any treatment), respectively. In 0.2% PAAA, the concentrations of hydrogen peroxide (H O ) and nitrate anions were directly proportional to the plasma discharge time, whilst nitrite anion (NO ) was not detected. However, the pH values of both 0.2% PAAA and plasma-activated water were inversely proportional to the plasma discharge time. Treatment with catalase, L-histidine, D-mannitol and sodium azide inhibited the antibacterial activity of PAAA.
H O , singlet oxygen, hydroxyl radical and NO are involved in the generation and decomposition of peroxynitrous acid generated from PAAA functioned as intermediate agent, which could diffuse through cell membranes of bacteria and induce cell injury.
This study provides the understanding of efficacy and selectivity of PAAA which could be a novel decontamination agent.
本研究旨在考察等离子体活化乙酸（PAAA）对鼠伤寒沙门氏菌细胞和生物膜的灭活效果，并阐明潜在的化学灭活途径。
通过向20 ml 0.2%（v/v）乙酸（AA）中放电等离子体20分钟（2.2 kHz和8.4 kVpp）制备PAAA。与对照组（未进行任何处理）相比，用0.2% PAAA和0.4% PAAA处理10分钟后，细胞和生物膜的数量分别减少了5.71 log CFU/ml和4 log CFU/cm²。在0.2% PAAA中，过氧化氢（H₂O₂）和硝酸根阴离子的浓度与等离子体放电时间成正比，而未检测到亚硝酸根阴离子（NO₂⁻）。然而，0.2% PAAA和等离子体活化水的pH值与等离子体放电时间成反比。用过氧化氢酶、L-组氨酸、D-甘露醇和叠氮化钠处理可抑制PAAA的抗菌活性。
H₂O₂、单线态氧、羟基自由基和NO₂⁻参与了作为中间剂的PAAA产生的过氧亚硝酸的生成和分解，其可通过细菌细胞膜扩散并诱导细胞损伤。
本研究提供了对PAAA功效和选择性的理解，PAAA可能是一种新型去污剂。
