Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia 5756151818, Iran.
Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia 5756151818, Iran.
J Food Prot. 2024 Sep;87(9):100336. doi: 10.1016/j.jfp.2024.100336. Epub 2024 Jul 27.
Biofilms are highly resistant to disinfectants and antimicrobials and are known as the primary source of food contamination. Salmonella Typhimurium (S. Typhimurium) and Staphylococcus aureus (S. aureus) have an excellent ability to form biofilm. This study aimed to evaluate the antibiofilm activity of ozonated water (O), acetic acid (AA), and lactic acid (LA), individually and sequentially, against biofilms of S. Typhimurium and S. aureus formed on the polystyrene surfaces. The antibiofilm effects of the treatments were evaluated using crystal violet staining and the viable count determination methods. In the staining method, the highest percentage of biofilm mass reduction was induced by successive use of ozonated water and acetic acid (O-AA), which reduced S. aureus biofilm mass by 44.36%. The sequential use of ozonated water and lactic acid (O-LA) could decrease S. Typhimurium biofilm mass by 57.26%. According to the viable count method, the most effective treatment was the sequential use of ozonated water and lactic acid (O-LA), which reduced S. aureus and S. Typhimurium biofilms by 1.76 and 4.06 log, respectively. It was concluded that the sequential use of ozonated water and organic acids can be considered a practical and environmentally friendly approach to control biofilms.
生物膜对消毒剂和抗菌剂具有很强的抵抗力，是食物污染的主要来源。肠炎沙门氏菌（S. Typhimurium）和金黄色葡萄球菌（S. aureus）形成生物膜的能力极强。本研究旨在评估臭氧水（O）、乙酸（AA）和乳酸（LA）单独和连续使用对聚苯乙烯表面形成的 S. Typhimurium 和 S. aureus 生物膜的抗生物膜活性。使用结晶紫染色和活菌计数法评估处理的抗生物膜效果。在染色法中，臭氧水和乙酸的连续使用（O-AA）诱导的生物膜质量减少百分比最高，可使金黄色葡萄球菌生物膜质量减少 44.36%。臭氧水和乳酸的连续使用（O-LA）可使肠炎沙门氏菌生物膜质量减少 57.26%。根据活菌计数法，最有效的处理方法是臭氧水和乳酸的连续使用（O-LA），可使金黄色葡萄球菌和肠炎沙门氏菌生物膜分别减少 1.76 和 4.06 log。结论是，臭氧水和有机酸的连续使用可以被认为是一种实用且环保的控制生物膜的方法。
