Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
Crit Rev Food Sci Nutr. 2023 Nov;63(33):11734-11749. doi: 10.1080/10408398.2022.2095973. Epub 2022 Aug 2.
Food-borne mycotoxins is one of the food safety concerns in the world. At present, nanosensors are widely used in the detection and analysis of mycotoxins due to their high specificity and sensitivity. In nanosensor-based mycotoxindetections, the sensitivity is mainly improved from two aspects. On the one hand, based on the principle of immune response, antigens and antibodies can be modified and developed. Such as single-domain heavy chain antibodies, aptamers, peptides, and antigen mimotopes. On the other hand, improvements and innovations have been made on signal amplification materials, including gold nanoparticles (AuNPs), quantum dots, and graphene, etc. Among them, gold nanoparticles can not only be used as a signal amplification material, but also can be used as carriers for identification elements, which can be used for signal amplification in detection. In this article, we systematically summarized the emerging strategies for enhancing the detection sensitivity of traditional gold nanoparticles-based nanosensors, in terms of recognition elements and signal amplification. Representative examples were selected to illustrate the potential mechanism of each strategy in enhancing the colorimetric signal intensity of AuNP and its potential application in biosensing. Finally, our review suggested the challenges and future prospects of gold particles in detection of mycotoxins.
食源性真菌毒素是全球食品安全关注的问题之一。由于具有高特异性和灵敏度，纳米传感器在真菌毒素的检测和分析中得到了广泛应用。在基于纳米传感器的真菌毒素检测中，灵敏度主要从两个方面得到提高。一方面，基于免疫反应原理，可以对抗原和抗体进行修饰和开发。例如单域重链抗体、适体、肽和抗原模拟肽。另一方面，对信号放大材料进行了改进和创新，包括金纳米粒子（AuNPs）、量子点和石墨烯等。其中，金纳米粒子不仅可以用作信号放大材料，还可以用作识别元件的载体，可用于检测中的信号放大。本文从识别元件和信号放大两个方面，系统地总结了增强传统基于金纳米粒子的纳米传感器检测灵敏度的新兴策略。选择了有代表性的例子来说明每种策略增强 AuNP 比色信号强度的潜在机制及其在生物传感中的潜在应用。最后，我们的综述提出了金纳米粒子在真菌毒素检测中的挑战和未来展望。
