Hu Weihua, Chen Hongming, Zhang Huanhuan, He Guangli, Li Xin, Zhang Xiaoxing, Liu Yang, Li Chang Ming
Institute for Clean Energy & Advanced Materials, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China; Faculty of Materials and Energy, Southwest University, Chongqing 400715, China; State Key Laboratory of Power Transmission Equipment & System Security and New Technology, Chongqing University, Chongqing 400044, China.
Institute for Clean Energy & Advanced Materials, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China; Faculty of Materials and Energy, Southwest University, Chongqing 400715, China.
J Colloid Interface Sci. 2014 Oct 1;431:71-6. doi: 10.1016/j.jcis.2014.06.007. Epub 2014 Jun 17.
Detection of multiple toxic mycotoxins is of importance in food quality control. Surface plasmon resonance imaging (SPRi) is an advanced tool for simultaneously multiple detections with accuracy; however, it suffers from limited sensitivity due to the instrumental constraint and small sizes of mycotoxins with only one epitope for an insensitive competitive immunoassay. In this work a gold nanoparticle (AuNP)-enhanced SPRi chip is designed to sensitively detect multiple mycotoxins using a competitive immunoassay format. The sensing surface is constructed by uniformly attaching dense mycotoxin antigens on poly[oligo(ethylene glycol) methacrylate-co-glycidyl methacrylate] (POEGMA-co-GMA) brush modified SPRi gold chip. After competitive binding in a sample solution containing respective monoclonal antibodies, secondary antibody-conjugated AuNPs are employed to bind with the captured monoclonal antibodies for further amplification of the SPRi signal. Highly specific and sensitive simultaneous detection is achieved for three typical mycotoxins including Aflatoxin B1 (AFB1), Ochratoxin A (OTA) and Zearalenone (ZEN) with low detection limits of 8, 30 and 15 pg mL(-1) and dynamic ranges covering three orders of magnitude.
多种有毒霉菌毒素的检测在食品质量控制中具有重要意义。表面等离子体共振成像（SPRi）是一种先进的工具，可同时进行多种准确检测；然而，由于仪器限制以及霉菌毒素尺寸小且只有一个表位用于不敏感的竞争免疫测定，其灵敏度有限。在这项工作中，设计了一种金纳米颗粒（AuNP）增强的SPRi芯片，采用竞争免疫测定形式灵敏地检测多种霉菌毒素。传感表面通过将致密的霉菌毒素抗原均匀附着在聚[聚（乙二醇）甲基丙烯酸酯 - 共 - 甲基丙烯酸缩水甘油酯]（POEGMA - 共 - GMA）刷修饰的SPRi金芯片上构建而成。在含有各自单克隆抗体的样品溶液中进行竞争结合后，使用与二抗偶联的AuNP与捕获的单克隆抗体结合，以进一步放大SPRi信号。对于三种典型的霉菌毒素，即黄曲霉毒素B1（AFB1）、赭曲霉毒素A（OTA）和玉米赤霉烯酮（ZEN），实现了高特异性和灵敏的同时检测，检测限低至8、30和15 pg mL(-1)，动态范围涵盖三个数量级。
