Dental School, University of Pernambuco (FOP/UPE), Avenida Professor Luís Freire, 700, Cidade Universitária, Recife, PE, 50740-540, Brazil.
Dental School, University of Pernambuco (Campus Arcoverde), Estrada Para Sítio Deserto, Arcoverde, PE, 1940, Brazil.
Clin Oral Investig. 2022 Nov;26(11):6663-6670. doi: 10.1007/s00784-022-04624-5. Epub 2022 Aug 2.
To analyze and compare, in vitro, the microhardness, sorption, solubility, color stability, and cytotoxicity of three types of resin composites: self-adhesive (SARC) (Dyad Flow (DF)/Kerr), bulk-fill (Filtek Bulk Fill Flow (FBF)/3 M ESPE), and conventional (Filtek Z350XT Flow (Z350)/3 M ESPE).
Thirty cylindrical specimens were prepared using a split metal mold (15 mm × 1 mm), divided into 3 groups (n = 10) according to the material used. Vickers hardness (VH) was calculated from three indentations (300gf/15 s) per specimen. The sorption and solubility were measured according to the ISO 4049:2009 specification after storing in distilled water for 7 days. The color of each resin composite was measured using a portable digital spectrophotometer according to the CIELAB system. After a 7-day immersion in coffee, the color variation (∆E) was calculated. Following the ISO 10993:2012, the cytotoxicity in Vero cells was evaluated through the MTT assay. The results were analyzed using the Kruskal-Wallis test to compare the studied groups. The Wilcoxon test was used to compare the assessments in each studied group. For cytotoxicity analysis, the data were compared by the ANOVA test (α = 0.05).
DF showed the lowest VH (28.67), highest sorption (0.543 µg/mm) and solubility (1.700 µg/mm), and higher ∆E after 7 days of coffee immersion (p = 0.008). The resin composites studied were considered non-cytotoxic.
The SARC presented inferior mechanical and physical-chemical properties than bulk-fill and conventional resin composites, with comparable cytotoxicity against Vero cells.
The simplification of the clinical protocol of SARC can minimize the number of possible failures during the restorative technique. However, considering their inferior physical and mechanical properties, their coverage with materials of higher mechanical properties and physical-chemical stability should be considered.
体外分析和比较三种树脂复合材料的显微硬度、吸附、溶解度、颜色稳定性和细胞毒性：自粘接型（SARC）（Dyad Flow（DF）/ Kerr）、块状填充型（Filtek Bulk Fill Flow（FBF）/ 3M ESPE）和传统型（Filtek Z350XT Flow（Z350）/ 3M ESPE）。
使用分体金属模具（15mm×1mm）制备 30 个圆柱形样本，根据所用材料将其分为 3 组（n=10）。对每个样本的三个压痕（300gf/15s）计算维氏硬度（VH）。根据 ISO 4049:2009 规范，在蒸馏水中储存 7 天后测量吸附和溶解度。使用便携式数字分光光度计根据 CIELAB 系统测量每种树脂复合材料的颜色。在咖啡中浸泡 7 天后，计算颜色变化（∆E）。根据 ISO 10993:2012，通过 MTT 测定法评估 Vero 细胞的细胞毒性。使用 Kruskal-Wallis 检验分析结果，以比较研究组。使用 Wilcoxon 检验比较每个研究组的评估结果。对于细胞毒性分析，使用方差分析（α=0.05）比较数据。
DF 显示出最低的 VH（28.67）、最高的吸附（0.543µg/mm）和溶解度（1.700µg/mm），以及在咖啡浸泡 7 天后更高的 ∆E（p=0.008）。研究的树脂复合材料被认为是非细胞毒性的。
与块状填充型和传统型树脂复合材料相比，SARC 的机械和物理化学性能较差，但对 Vero 细胞的细胞毒性相当。
SARC 临床方案的简化可以最大限度地减少修复技术过程中可能出现的故障数量。然而，考虑到其较差的物理和机械性能，应考虑使用具有更高机械性能和物理化学稳定性的材料进行覆盖。
