Predheepan Dhakshanya, Daddangadi Akshatha, Uppangala Shubhashree, Laxminarayana Sindhura Lakshmi Koulmane, Raval Keyur, Kalthur Guruprasad, Kovačič Borut, Adiga Satish Kumar
Division of Clinical Embryology, Department of Reproductive Science, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal 576104, India.
Division of Reproductive Genetics, Department of Reproductive Science, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal 576104, India.
Endocrinology. 2022 Sep 1;163(9). doi: 10.1210/endocr/bqac121.
Reproductive abnormalities in women with a history of childhood diabetes are believed to be partially attributed to hyperglycemia. Prolonged hyperglycemia can negatively affect ovarian function and fertility during reproductive life. To address this in an experimental setting, the present study used streptozotocin-induced hyperglycemic prepubertal mouse model. The impact of prolonged hyperglycemic exposure during prepubertal life on ovarian function, oocyte quality, and functional competence was assessed in adult mice. The ovarian reserve was not significantly altered; however, the in vitro maturation potential (P < 0.001), mitochondrial integrity (P < 0.01), and meiotic spindle assembly (P < 0.05-0.001) in oocytes were significantly affected in hyperglycemic animals in comparison to control groups. The results from the study suggest that prepubertal hyperglycemia can have adverse effects on the oocyte functional competence and spindle integrity during the reproductive phase of life. Because these changes can have a significant impact on the genetic integrity and developmental potential of the embryos and fetus, the observation warrants further research both in experimental and clinical settings.
有儿童期糖尿病病史的女性出现生殖异常被认为部分归因于高血糖。长期高血糖会对生殖期的卵巢功能和生育能力产生负面影响。为了在实验环境中解决这个问题，本研究使用链脲佐菌素诱导的青春期前高血糖小鼠模型。在成年小鼠中评估了青春期前长期高血糖暴露对卵巢功能、卵母细胞质量和功能能力的影响。卵巢储备没有显著改变；然而，与对照组相比，高血糖动物卵母细胞的体外成熟潜力（P < 0.001）、线粒体完整性（P < 0.01）和减数分裂纺锤体组装（P < 0.05 - 0.001）受到显著影响。该研究结果表明，青春期前高血糖会对生命生殖阶段的卵母细胞功能能力和纺锤体完整性产生不利影响。由于这些变化会对胚胎和胎儿的遗传完整性和发育潜力产生重大影响，这一观察结果值得在实验和临床环境中进一步研究。
