Department of Food Quality Control and Analysis, Muş Alparslan University, Muş, Turkey.
Department of Medical Biology, Ankara Medipol University, Ankara, Turkey.
Anal Biochem. 2022 Oct 1;654:114825. doi: 10.1016/j.ab.2022.114825. Epub 2022 Jul 30.
This study aimed to reveal the intermittent fasting-induced alterations in biomolecules of the liver, ileum, and colon tissues of rats using Support Vector Machine (SVM) and Linear Discriminant Analysis (LDA) algorithms developed on infrared spectrochemical data. LDA prediction accuracies were generally calculated in the range of 95-100%, while training and validation accuracies of SVM were in the range of 91-100% and 83-91%, respectively. The quantitative measurements of spectral bands at the CH (lipids), Amide (proteins), and PO antisymmetric (nucleic acids) stretching regions were performed to monitor modulated metabolic processes. The concentration of biomolecules and phosphorylation rate of proteins were found higher in studied tissues. The altered conformations and low rates of carbonylation (oxidation) were also common in proteins. No significant change was recorded for the length of fatty acid acyl chains (A/A band area ratio) in the liver, whereas the shortening of acyl chains was calculated as 23% and 27% in ileum and colon tissues, respectively. Enhanced membrane dynamics (Bw/Bw bandwidth ratio) were depicted in the liver (35% increase), while a decline in dynamics was apparent in the ileum (36% decrease) and colon (31% decrease). The study revealed important alterations in major biomolecules of studied tissues.
本研究旨在利用支持向量机（SVM）和线性判别分析（LDA）算法，揭示间歇禁食对大鼠肝脏、回肠和结肠组织中生物分子的影响。LDA 预测准确率一般在 95-100%之间，而 SVM 的训练和验证准确率分别在 91-100%和 83-91%之间。对 CH（脂质）、酰胺（蛋白质）和 PO 反对称（核酸）伸缩区域的光谱带进行定量测量，以监测调节代谢过程。在研究的组织中，生物分子的浓度和蛋白质的磷酸化率较高。蛋白质的构象改变和低羰基化（氧化）速率也很常见。肝脏中脂肪酸酰链长度（A/A 带面积比）没有明显变化，而回肠和结肠组织中酰链的缩短分别计算为 23%和 27%。肝脏中增强的膜动力学（Bw/Bw 带宽比）增加了 35%，而回肠中动力学的下降则更为明显（下降了 36%），结肠中动力学的下降（下降了 31%）。研究揭示了研究组织中主要生物分子的重要变化。
