Department of Medical Biology and Genetics, Ankara Medipol University, Ankara, Turkey.
Department of Molecular Biology and Genetics, Muş Alparslan University, Muş, Turkey.
J Cell Mol Med. 2024 Jan;28(1):e18014. doi: 10.1111/jcmm.18014. Epub 2023 Oct 28.
This study aimed to examine the impact of SCD Probiotics supplementation on liver biomolecule content and histological changes during a 30-day intermittent fasting (IF) program in 24-month-old male Sprague-Dawley rats. Rats underwent 18-h daily fasting and received 1 × 10 CFU of SCD Probiotics daily. Liver tissue biomolecules were analysed using FTIR Spectroscopy, LDA, and SVM techniques, while histopathological evaluations used Haematoxylin and eosin and Masson trichrome-stained tissues. Blood samples were collected for biochemical analysis. Gross alterations in the quantity of biomolecules were observed with individual or combined treatments. LDA and SVM analyses demonstrated a high accuracy in differentiating control and treated groups. The combination treatments led to the most significant reduction in cholesterol ester (1740 cm ) and improved protein phosphorylation (A /A and A /A ) and carbonylation (A /A ). Individually, IF and SCD Probiotics were more effective in enhancing membrane dynamics (Bw /Bw ). In treated groups, histological evaluations showed decreased hepatocyte degeneration, lymphocyticinfiltration, steatosis and fibrosis. Serum ALP, LDH and albumin levels significantly increased in the SCD Probiotics and combined treatment groups. This study offers valuable insights into the potential mechanisms behind the beneficial effects of IF and SCD Probiotics on liver biomolecule content, contributing to the development of personalized nutrition and health strategies.
本研究旨在探讨 SCD 益生菌补充对 24 月龄雄性 Sprague-Dawley 大鼠 30 天间歇性禁食 (IF) 方案中肝脏生物分子含量和组织学变化的影响。大鼠接受每日 18 小时禁食，并每天接受 1×10 CFU 的 SCD 益生菌。使用傅里叶变换红外光谱 (FTIR) 光谱、LDA 和 SVM 技术分析肝组织生物分子，使用苏木精和伊红 (H&E) 和 Masson 三色染色组织进行组织病理学评估。收集血液样本进行生化分析。单独或联合处理观察到生物分子数量的个体变化。LDA 和 SVM 分析表明，区分对照和处理组的准确率很高。联合处理导致胆固醇酯 (1740 cm ) 的降低最为显著，同时改善了蛋白质磷酸化 (A/A 和 A/A ) 和羰基化 (A/A )。单独的 IF 和 SCD 益生菌更有效地增强了膜动力学 (Bw/Bw )。在处理组中，组织学评估显示肝细胞变性、淋巴细胞浸润、脂肪变性和纤维化减少。SCD 益生菌和联合治疗组的血清碱性磷酸酶 (ALP)、乳酸脱氢酶 (LDH) 和白蛋白水平显著升高。本研究为 IF 和 SCD 益生菌对肝脏生物分子含量的有益影响的潜在机制提供了有价值的见解，有助于个性化营养和健康策略的发展。
