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裸裂尻鱼基因组的染色体水平组装。

Chromosome-level assembly of Gymnocypris eckloni genome.

机构信息

State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, 810016, China.

College of Eco-Environmental Engineering, Qinghai University, Xining, 810016, China.

出版信息

Sci Data. 2022 Aug 2;9(1):464. doi: 10.1038/s41597-022-01595-w.

Abstract

Gymnocypris eckloni is widely distributed in isolated lakes and the upper reaches of the Yellow River and play significant roles in the trophic web of freshwater communities. In this study, we generated a chromosome-level genome of G. eckloni using PacBio, Illumina and Hi-C sequencing data. The genome consists of 23 pseudo-chromosomes that contain 918.68 Mb of sequence, with a scaffold N50 length of 43.54 Mb. In total, 23,157 genes were annotated, representing 94.80% of the total predicted protein-coding genes. The phylogenetic analysis showed that G. eckloni was most closely related to C. carpio with an estimated divergence time of ~34.8 million years ago. For G. eckloni, we identified a high-quality genome at the chromosome level. This genome will serve as a valuable genomic resource for future research on the evolution and ecology of the schizothoracine fish in the Qinghai-Tibetan Plateau.

摘要

黄河高原鳅广泛分布于孤立湖泊和黄河上游,在淡水生物群落的营养网中发挥着重要作用。本研究利用 PacBio、Illumina 和 Hi-C 测序数据生成了黄河高原鳅的染色体水平基因组。该基因组由 23 条假染色体组成,包含 918.68 Mb 的序列,支架 N50 长度为 43.54 Mb。共注释了 23157 个基因,代表了总预测蛋白编码基因的 94.80%。系统发育分析表明,黄河高原鳅与鲤鱼的亲缘关系最近,估计分化时间约为 3480 万年前。对于黄河高原鳅,我们在染色体水平上鉴定出了一个高质量的基因组。该基因组将成为未来青藏高原鲇形目鱼类进化和生态学研究的宝贵基因组资源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f69c/9346132/aedee0eb7912/41597_2022_1595_Fig1_HTML.jpg

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