Key Laboratory of Plateau Wetland Ecology and Environmental Protection, Xichang University, Xichang 615013, China.
Genes (Basel). 2024 Jan 29;15(2):182. doi: 10.3390/genes15020182.
Artificial hybrid breeding can optimize parental traits to cultivate excellent hybrids with enhanced economic value. In this study, we investigated the growth performance and transcriptomes of (♀) and (♂) and their F1 hybrid fishes. Hatched individuals of (GP) and (GE) of the same size and their F1 hybrids (GH) were separately cultured for eight months in three cement tanks ( = 3). The growth indexes were measured, which showed that the growth rate of the groups was GE > GH > GP, while the survival rate was GH > GE > GP. The RNA-Seq data analysis of the muscles from the three fish strains revealed that gene transcription has a significant impact on F1 hybrid fish and its parents. The differentially expressed genes (DEGs) in GH show less differences with GP, but more with GE. qRT-PCR was used to confirm the expression profiles of the chosen DEGs, and the results showed positive correlations with the RNA-seq data. KEGG enrichment results indicated that the DEGs were related to a variety of molecular functions, such as glycolysis/gluconeogenesis, arachidonic acid formation, citrate cycle, and the MAPK, PI3K-Akt, or mTOR signal pathways. Subsequent analysis indicated that there may be a significant correlation between the differential expression of IGF2 and a difference in the growth of GE and GP.
人工杂交育种可以优化亲本性状，培育具有更高经济价值的优良杂种。本研究调查了 （♀）和 （♂）及其 F1 杂交鱼的生长性能和转录组。将大小相同的 （GP）和 （GE）的孵化个体及其 F1 杂种（GH）分别在三个水泥池中（ = 3）养殖八个月。测量了生长指标，结果表明，生长速度为 GE > GH > GP，而存活率为 GH > GE > GP。来自三种 鱼系肌肉的 RNA-Seq 数据分析表明，基因转录对 F1 杂交鱼及其亲本有显著影响。GH 中的差异表达基因（DEGs）与 GP 的差异较小，但与 GE 的差异较大。qRT-PCR 用于验证所选 DEGs 的表达谱，结果与 RNA-seq 数据呈正相关。KEGG 富集结果表明，DEGs 与多种分子功能有关，如糖酵解/糖异生、花生四烯酸形成、柠檬酸循环以及 MAPK、PI3K-Akt 或 mTOR 信号通路。后续分析表明，IGF2 的差异表达与 GE 和 GP 生长差异之间可能存在显著相关性。
