Sun Qiuyu, Liu Jie, Huang Chong, Liu Xiufeng, Gao Jianmeng, Li Leifu, Luo Yong, Ma Zhanhong
Department of Plant Pathology, China Agricultural University, Beijing 100193, China.
National Agro-tech Extension and Service Center, Beijing 100125, China.
Phytopathology. 2023 Jan;113(1):21-30. doi: 10.1094/PHYTO-06-21-0256-R. Epub 2023 Jan 12.
Southern corn rust (SCR) caused by is one of the most devastating diseases in the world. In recent years, SCR has been upgraded from a minor to a major disease around the world, including in China. However, little is known about its population genetics and structure in China. In this study, we analyzed 288 isolates collected from various localities during 2017 in seven Chinese provinces: Guangxi, Guangdong, Anhui, Hunan, Shandong, Henan, and Shaanxi. The isolates were analyzed using nine microsatellite markers. The population structure, genetic diversity, and reproduction mode of were investigated based on genotype data. Strong genotypic diversity was detected and clonal reproduction was dominant. The populations collected from the pathogen's winter-reproductive regions harbored more genotypes than those collected from the pathogen's epidemic regions. The spatial differences in genotypic richness, and evenness among the populations were significant, and showed a decreasing trend from south to north. Most isolates were clustered into two clonal groups. Two high-frequency multilocus genotypes (MLGs), MLG1 and MLG2, were widely distributed in all populations. Our analyses confirmed that employed clone dispersal from the pathogen's winter-reproductive regions to the pathogen's epidemic regions, and in addition to the sources from the pathogen's winter-reproductive regions, the pathogen in Anhui and Hunan might also have other sources from areas such as Taiwan, China, or/and Southeast Asia, and the pathogen went through a genetic bottleneck during its dispersal. These findings provide initial insights into the reproduction mode and dispersal pathways of in China.
由[病原菌名称未给出]引起的南方玉米锈病（SCR）是世界上最具毁灭性的病害之一。近年来，SCR在全球范围内，包括在中国，已从一种次要病害升级为主要病害。然而，关于其在中国的群体遗传学和结构知之甚少。在本研究中，我们分析了2017年从中国七个省份（广西、广东、安徽、湖南、山东、河南和陕西）不同地点收集的288个分离株。使用九个微卫星标记对这些分离株进行分析。基于基因型数据研究了[病原菌名称未给出]的群体结构、遗传多样性和繁殖模式。检测到强烈的基因型多样性，且克隆繁殖占主导地位。从病原菌越冬繁殖区收集的群体比从病原菌流行区收集的群体具有更多的基因型。群体间基因型丰富度和均匀度的空间差异显著，呈现出从南到北递减的趋势。大多数分离株聚为两个克隆群。两个高频多位点基因型（MLGs），即MLG1和MLG2，在所有群体中广泛分布。我们的分析证实，[病原菌名称未给出]通过克隆传播从病原菌越冬繁殖区扩散到病原菌流行区，并且除了来自病原菌越冬繁殖区的来源外，安徽和湖南的病原菌可能还存在来自中国台湾或/和东南亚等地区的其他来源，且病原菌在扩散过程中经历了遗传瓶颈。这些发现为[病原菌名称未给出]在中国的繁殖模式和传播途径提供了初步见解。
