精子冷冻保存对小鼠精子活力和前AKAP4浓度的影响。

Influence of sperm cryopreservation on sperm motility and proAKAP4 concentration in mice.

作者信息

Boersma Auke, Primus Jasmin, Wagner Bettina, Broukal Veronika, Andersen Lill, Pachner Barbara, Dahlhoff Maik, Rülicke Thomas, Auer Kerstin E

机构信息

Institute of in vivo and in vitro Models University of Veterinary Medicine Vienna Vienna Austria.

Department of Radiology Charité - Universitätsmedizin Berlin Berlin Germany.

出版信息

Reprod Med Biol. 2022 Jul 29;21(1):e12480. doi: 10.1002/rmb2.12480. eCollection 2022 Jan-Dec.

DOI:10.1002/rmb2.12480

PMID:35919386

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9336535/

Abstract

BACKGROUND

The protein proAKAP4 is crucial for sperm motility and has been suggested as an indicator of male fertility. We determined the relationship between proAKAP4 concentration and sperm motility parameters in mice, and investigated the effects of cryopreservation on these variables.

METHODS

Computer-assisted sperm analysis and ELISA were applied to determine sperm motility and proAKAP4 concentration in fresh and frozen-thawed epididymal sperm of SWISS, B6D2F1, C57BL/6N, and BALB/c mice.

RESULTS

ProAKAP4 levels ranged between 12 and 89 ng/ml and did not differ between fresh and frozen-thawed samples, or between strains. We found a negative relationship between proAKAP4 levels and some sperm motility parameters. Sperm traits differed between strains, and cryopreservation negatively affected sperm velocity but not sperm direction parameters.

CONCLUSION

ProAKAP4 levels in epididymal mouse spermatozoa were unaffected by cryopreservation, highlighting the robustness of this parameter as a potentially time-independent marker for sperm motility and fertility. The high individual variation in proAKAP4 levels supports the potential role of proAKAP4 as a marker for sperm quality, though we found no positive, and even negative relationships between proAKAP4 levels and some sperm motility parameters. Future studies have to investigate the significance of proAKAP4 as an indicator for fertility in mice.

摘要

背景

前体A激酶锚定蛋白4（proAKAP4）对精子活力至关重要，已被认为是男性生育能力的一个指标。我们确定了小鼠体内proAKAP4浓度与精子活力参数之间的关系，并研究了冷冻保存对这些变量的影响。

方法

应用计算机辅助精子分析和酶联免疫吸附测定法（ELISA）来测定瑞士小鼠、B6D2F1小鼠、C57BL/6N小鼠和BALB/c小鼠新鲜及冻融附睾精子的活力和proAKAP4浓度。

结果

proAKAP4水平在12至89纳克/毫升之间，新鲜样本与冻融样本之间以及不同品系之间均无差异。我们发现proAKAP4水平与一些精子活力参数之间呈负相关。不同品系的精子特征存在差异，冷冻保存对精子速度有负面影响，但对精子方向参数没有影响。

结论

附睾小鼠精子中的proAKAP4水平不受冷冻保存的影响，这突出了该参数作为精子活力和生育能力潜在的与时间无关的标志物的稳健性。尽管我们发现proAKAP4水平与一些精子活力参数之间没有正相关，甚至呈负相关，但proAKAP4水平的高度个体差异支持了其作为精子质量标志物的潜在作用。未来的研究必须调查proAKAP4作为小鼠生育能力指标的意义。