Magollah Tabu Mungia, Kim Hyo-Rim, Ko Ji-Yun, Park Su-Yeon, Kwon Seo-Yeon, Kim Yu-Kyeong, Lee Ji-Hyo, Seo Yong-Soo, Yang Ji-Young, Lee Yang-Bong
Department of Food Science and Technology, Pukyong National University, Busan 48513, Korea.
Central Laboratory, Pukyong National University, Busan 48513, Korea.
Prev Nutr Food Sci. 2022 Jun 30;27(2):234-240. doi: 10.3746/pnf.2022.27.2.234.
Ethyl carbamate (EC) is a known carcinogen, and therefore its intake is regulated internationally. The objectives of this study were to compare the EC recovery yields under different liquid-liquid extraction (LLE) conditions and to investigate the optimum conditions of the aqueous two-phase system (ATPS) for EC extraction. Our results showed that for the LLE method, addition of 15% NaCl improved the EC yield by 15%, and dichloromethane as the extraction solvent showed a slightly higher yield (about 5%) than chloroform. However, there was little difference in the yield when mixing was performed using an ultrasonic bath compared to a vortex mixer. Using response surface methodology with central composite design to analyze the ATPS results, optimal extraction was found to occur at 21.5°C for 2.8 h in the sample containing 70% alcohol and 15% phosphate, showing a recovery yield of 75.64%. This information can be applied to alcoholic beverages and other fermented food products to analyze EC with better extraction methods, depending on the types of food.
氨基甲酸乙酯（EC）是一种已知的致癌物，因此其摄入量在国际上受到监管。本研究的目的是比较不同液液萃取（LLE）条件下的EC回收率，并研究用于EC萃取的双水相系统（ATPS）的最佳条件。我们的结果表明，对于LLE方法，添加15%的氯化钠可使EC产率提高15%，以二氯甲烷作为萃取溶剂时的产率略高于氯仿（约5%）。然而，与使用涡旋混合器相比，使用超声浴进行混合时产率差异不大。采用中心复合设计的响应面法分析ATPS结果，发现在含有70%乙醇和15%磷酸盐的样品中，在21.5°C下萃取2.8小时可实现最佳萃取，回收率为75.64%。根据食品类型，这些信息可应用于酒精饮料和其他发酵食品，以采用更好的萃取方法分析EC。
