Universidade Federal Rural de Pernambuco, Departamento de Morfologia e Fisiologia Animal, Rua Dom Manuel de Medeiros, s/n, Dois Irmãos, 52171-300 Recife, PE, Brazil.
Universidade Federal do Piauí, Departamento de Nutrição, Rua Cícero Eduardo, s/n, Junco, 64600-000 Picos, PI, Brazil.
An Acad Bras Cienc. 2022 Aug 1;94(3):e20211297. doi: 10.1590/0001-3765202220211297. eCollection 2022.
Angiogenesis (budding of new blood vessels) is involved in several processes, including the development of embryos and growth of tumors. Schinus terebinthifolia leaves express an antitumor lectin (SteLL). This work hypothesized that SteLL can interfere with the formation of a vascular network from preexisting vessels. To test this hypothesis, the effect of SteLL on the angiogenesis process was assessed using an in vivo model of yolk sac membrane of Coturnix japonica embryos. SteLL was isolated with purification factor of 46.6. As expected, polyacrylamide gel electrophoresis (PAGE) for native basic proteins confirmed the homogeneity and PAGE in presence of dodecyl sodium sulphate revealed a single 14-kDa polypeptide band. The fractal analysis by box counting and information dimension measurements indicated that SteLL at 1.35 mg/mL significantly decreased by ca. 12% the angiogenesis within the C. japonica yolk sac membrane regarding the control. The inhibition of the vascular network formation in the yolk sac membrane resulted in decreased blood supply to the embryos. Consequently, the area of embryos was significantly reduced by 9.2% regarding the control, which corroborated with the antiangiogenic activity of SteLL. The findings implicate SteLL as an antiangiogenic agent and add to the panel of biological activities of this lectin.
血管生成（新血管的萌芽）涉及多种过程，包括胚胎发育和肿瘤生长。巴西栗叶表达一种抗肿瘤凝集素（SteLL）。本研究假设 SteLL 可以干扰从现有血管形成血管网络的过程。为了验证这一假设，使用 Coturnix japonica 胚胎蛋黄膜的体内模型评估了 SteLL 对血管生成过程的影响。SteLL 用 46.6 的纯化因子进行分离。正如预期的那样，用于天然碱性蛋白的聚丙烯酰胺凝胶电泳（PAGE）证实了其均一性，而在十二烷基硫酸钠存在下的 PAGE 则显示出单一的 14 kDa 多肽带。分形分析的盒子计数和信息维数测量表明，SteLL 在 1.35 mg/mL 时，与对照组相比，C. japonica 蛋黄膜中的血管生成显著降低了约 12%。蛋黄膜中血管网络的形成受到抑制，导致胚胎的血液供应减少。因此，胚胎的面积与对照组相比显著减少了 9.2%，这与 SteLL 的抗血管生成活性一致。这些发现表明 SteLL 是一种抗血管生成剂，并为该凝集素的多种生物学活性增加了一个新的内容。
