de Oliveira Marinho Amanda, Alves da Costa Jainaldo, Silva Dos Santos Alícia Natalie, Cavalcanti de Barros Matheus, Pimentel Carynne do Nascimento, Arnaldo da Silva Anderson, Guedes Paiva Patrícia Maria, Napoleão Thiago Henrique, Leite de Siqueira Patriota Leydianne
Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
Departamento de Anatomia, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
J Ethnopharmacol. 2024 Oct 28;333:118496. doi: 10.1016/j.jep.2024.118496. Epub 2024 Jun 25.
Schinus terebinthifolia Raddi (Anacardiaceae), known as Brazilian pepper tree, stands out as a medicinal plant widely used in traditional medicine. The leaves are popularly used as anti-inflammatory agent and to relieve inflammatory conditions such as bronchitis, ulcers, and wounds, for example.
The present study evaluated the acute toxicity, genotoxicity, and anti-inflammatory activity of S. terebinthifolia leaf lectin (SteLL) in mice (Mus musculus).
In the acute toxicity assay, the animals were treated intraperitoneally (i.p.) or orally (per os) with a single dose of 100 mg/kg. Genotoxicity was assessed by the comet and micronucleus assays. Carrageenan-induced peritonitis and paw edema models were used to evaluate the anti-inflammatory effects of SteLL (1, 5 and 10 mg/kg, i.p.).
No animal died and no signs of intoxication or histopathological damage were observed in the acute toxicity assay. Genotoxic effect was not detected. In peritonitis assay, SteLL reduced in 56-69% leukocyte migration to the peritoneal cavity; neutrophil count decreased by 25-32%, while mononuclear cell count increased by 67-74%. SteLL promoted a notable reduction of paw edema after 4 h (61.1-63.4%). Morphometric analysis showed that SteLL also decreased the thickness of epidermal edema (30.2-40.7%). Furthermore, SteLL decreased MPO activity, plasma leakage, NO release, and modulated cytokines in both peritoneal fluid and paw homogenate.
SteLL did not induce acute toxicity or genotoxicity in mice and stands out as a promising candidate in the development of new phytopharmaceuticals with anti-inflammatory action.
胡椒树（漆树科），又称巴西胡椒树，是一种在传统医学中广泛使用的药用植物。例如，其叶子通常用作抗炎剂，用于缓解支气管炎、溃疡和伤口等炎症。
本研究评估了胡椒树叶凝集素（SteLL）对小鼠（小家鼠）的急性毒性、遗传毒性和抗炎活性。
在急性毒性试验中，动物腹腔注射（i.p.）或口服（经口）单剂量100mg/kg。通过彗星试验和微核试验评估遗传毒性。采用角叉菜胶诱导的腹膜炎和爪肿胀模型评估SteLL（1、5和10mg/kg，腹腔注射）的抗炎作用。
在急性毒性试验中，没有动物死亡，也未观察到中毒迹象或组织病理学损伤。未检测到遗传毒性作用。在腹膜炎试验中，SteLL使白细胞向腹腔的迁移减少了56 - 69%；中性粒细胞计数减少了25 - 32%，而单核细胞计数增加了67 - 74%。SteLL在4小时后使爪肿胀显著减轻（61.1 - 63.4%）。形态计量分析表明，SteLL还降低了表皮水肿的厚度（30.2 - 40.7%）。此外，SteLL降低了腹膜液和爪匀浆中的MPO活性、血浆渗漏、NO释放，并调节了细胞因子。
SteLL在小鼠中未诱导急性毒性或遗传毒性，是开发具有抗炎作用的新型植物药的有前景的候选物。
