Sarli Dinorah Anabella, Gomes Gisele Teixeira, Reznikov Sebastián, Acosta Federico Francisco, Fariña Julia Inés, Delgado Osvaldo Daniel, Sánchez Leandro A
Planta Piloto de Procesos Industriales Microbiológicos (PROIMI)-CONICET, San Miguel de Tucumán, Argentina.
Estación Experimental Agroindustrial Obispo Colombres (EEAOC), San Miguel de Tucumán, Argentina.
J Appl Microbiol. 2022 Nov;133(5):2835-2850. doi: 10.1111/jam.15759. Epub 2022 Aug 9.
The aim was to characterize cold-adapted bacteria by testing their PGP features and antagonistic activity against Macrophomina phaseolina, both in vitro and coating soybean seeds (Glycine max [L.] Merr.).
Burkholderia gladioli MB39, Serratia proteamaculans 136 and Serratia proteamaculans 137 were evaluated. In vitro tests showed that S. proteamaculans 136 and 137 produce siderophore and indole-acetic acid (IAA), solubilize phosphate and fix nitrogen. Additionally, B. gladioli MB39 and S. proteamaculans 137 showed hydrolase activity and potent antifungal effects. The biocontrol efficacy over soybean seeds was evaluated using in vitro and greenhouse methods by immersing seeds into each bacterial suspension. As a result, S. proteamaculans 136 has improved the performance in all the seed germination evaluated parameters. In addition, S. proteamaculans 137 and B. gladioli MB39 strongly inhibited M. phaseolina, reducing the infection index values to 10% and 0%, respectively.
Serratia proteamaculans 136, 137 and Burkholderia gladioli MB39 showed plant growth promotion features and inhibition of Macrophomina phaseolina infection by producing different antifungal compounds.
Our results reinforce the application of cold-adapted Serratia proteamaculans and Burkholderia gladioli bacterial strains as candidates for developing microbial formulation to promote plant growth and guarantee antifungal protection in soybean crops.
通过测试冷适应细菌的植物生长促进特性及其对菜豆壳球孢菌的拮抗活性，对其进行特性描述，测试在体外以及包衣大豆种子（大豆（Glycine max [L.] Merr.））上的情况。
对唐菖蒲伯克霍尔德菌MB39、黏质沙雷氏菌136和黏质沙雷氏菌137进行了评估。体外测试表明，黏质沙雷氏菌136和137产生铁载体和吲哚 - 乙酸（IAA），能溶解磷酸盐并固定氮。此外，唐菖蒲伯克霍尔德菌MB39和黏质沙雷氏菌137表现出水解酶活性和强大的抗真菌作用。通过将种子浸入每种细菌悬浮液中，采用体外和温室方法评估了对大豆种子的生物防治效果。结果，黏质沙雷氏菌136在所有评估的种子发芽参数方面表现有所改善。此外，黏质沙雷氏菌137和唐菖蒲伯克霍尔德菌MB39强烈抑制菜豆壳球孢菌，使感染指数值分别降至10%和0%。
黏质沙雷氏菌136、137和唐菖蒲伯克霍尔德菌MB39通过产生不同的抗真菌化合物表现出促进植物生长的特性并抑制菜豆壳球孢菌感染。
我们的结果加强了冷适应的黏质沙雷氏菌和唐菖蒲伯克霍尔德菌菌株作为开发促进植物生长并保证大豆作物抗真菌保护的微生物制剂候选菌株的应用。
