Department of Science, Faculty of Arts, Science and Commerce (FASC), Mody Institute of Technology & Science (MITS), Lakshmangarh, Sikar, Rajasthan, India.
Biotechnol Lett. 2011 Nov;33(11):2287-95. doi: 10.1007/s10529-011-0699-0. Epub 2011 Jul 16.
Charcoal rot disease, caused by the fungus Macrophomina phaseolina, leads to significant yield losses of soybean crops. One strategy to control charcoal rot is the use of antagonistic, root-colonizing bacteria. Rhizobacteria A(5)F and FPT(7)21 and Pseudomonas sp. strain GRP(3) were characterized for their plant growth-promotion activities against the pathogen. Rhizobacterium FPT(7)21 exhibited higher antagonistic activity against the pathogen on dual plate assay compared to strain A(5)F and GRP(3). FPT(7)21 and GRP(3) gave decreased disease intensity in terms of average number of pathogen-infested plants. Lipoxygenase (LOX), phenylalanine ammonia-lyase (PAL), and peroxidase (POD) activities were estimated in extracts of plants grown from seeds that were treated with rhizobacteria, and inoculated with spore suspension of M. phaseolina. The activity of these enzymes after challenge with the test pathogen increased. Strains FPT(7)21 and GRP(3) exhibited maximum increases in LOX, PAL and POD activity (U mg(-1) fresh leaf wt) compared to strain A(5)F.
炭腐病由真菌 Macrophomina phaseolina 引起，导致大豆作物产量显著下降。防治炭腐病的策略之一是使用具有拮抗作用、定殖于根部的细菌。对根际细菌 A(5)F 和 FPT(7)21 以及 Pseudomonas sp. strain GRP(3)进行了鉴定，以评估它们对病原菌的促生作用。在双层平板测定中，与 A(5)F 和 GRP(3)相比，FPT(7)21 对病原菌表现出更高的拮抗活性。与对照相比，FPT(7)21 和 GRP(3)处理的植株感病程度降低，表现在感病植株的平均数量减少。用含有根际细菌的种子种植的植物提取物中，测定了脂氧合酶 (LOX)、苯丙氨酸解氨酶 (PAL)和过氧化物酶 (POD)的活性，然后用 M. phaseolina 的孢子悬浮液进行接种。与测试病原菌接触后，这些酶的活性增加。与 A(5)F 相比，FPT(7)21 和 GRP(3)菌株的 LOX、PAL 和 POD 活性（U mg(-1)新鲜叶片重量）增加最多。
