重组 AcMNPV-gp64-EGFP 与协同增效剂磷酸三苯酯对斜纹夜蛾的高效防治作用。

Recombinant AcMNPV-gp64-EGFP and synergist triphenyl phosphate, an effective combination against Spodoptera frugiperda.

机构信息

Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006, China.

出版信息

Biotechnol Lett. 2022 Sep;44(9):1081-1096. doi: 10.1007/s10529-022-03286-3. Epub 2022 Aug 3.

DOI:10.1007/s10529-022-03286-3

PMID:35922646

Abstract

OBJECTIVES

AcMNPV is a kind of microbial insecticide that can significantly relieve the resistance of Spodoptera frugiperda to chemical pesticides. TPP is a widely used synergist, which can reduce the use of pesticides by inhibiting carboxylesterase. It is emergently needed to develop a biological control way of Spodoptera frugiperda.

RESULTS

GP64 mediates low-pH-triggered membrane fusion during entry by endocytosis and participates in AcMNPV particle budding. We explored the synergistic anti-insect activity of AcMNPV-gp64-EGFP and TPP. AcMNPV-gp64-EGFP could increase progeny virus proliferation and accelerate the transcription of 38k and vp39 genes. TPP could inhibit the carboxylesterase activity in the midgut of Spodoptera frugiperda larvae infected with AcMNPV-gp64-EGFP and enhance the virulence of AcMNPV-gp64-EGFP to Spodoptera frugiperda.

CONCLUSIONS

TPP targeted carboxylesterase inhibition so that AcMNPV-gp64-EGFP could escape the antiviral response in insect hosts. It provided a novel strategy for the prevention of Spodoptera frugiperda.

摘要

目的

AcMNPV 是一种微生物杀虫剂，能显著缓解斜纹夜蛾对化学农药的抗药性。TPP 是一种广泛使用的增效剂，它可以通过抑制羧酸酯酶来减少农药的使用。迫切需要开发一种防治斜纹夜蛾的生物防治方法。

结果

GP64 在进入细胞时通过内吞作用介导低 pH 触发的膜融合，并参与 AcMNPV 颗粒出芽。我们探讨了 AcMNPV-gp64-EGFP 和 TPP 的协同抗虫活性。AcMNPV-gp64-EGFP 可以增加子代病毒的增殖，并加速 38k 和 vp39 基因的转录。TPP 可以抑制感染 AcMNPV-gp64-EGFP 的斜纹夜蛾幼虫中肠的羧酸酯酶活性，增强 AcMNPV-gp64-EGFP 对斜纹夜蛾的毒力。