School of Resources Environment and Safety Engineering, University of South China, Hengyang, 421001, Hunan, People's Republic of China.
School of Chemistry and Chemical Engineering, University of South China, Hengyang, 421001, Hunan, People's Republic of China.
World J Microbiol Biotechnol. 2022 Aug 4;38(10):174. doi: 10.1007/s11274-022-03362-w.
Uptaking U(VI) from the environment by biological method is an environmental friendly and efficient way. In this work, we have acquired and isolated the protein DSR A by genetic engineering, then assessed its capacity and mechanisms to absorb U(VI) from wastewater. As results, we proved that protein DSR A can precisely recognize, enrich and remove uranyl ions in simulated wastewater solution. Its great removal potential was demonstrated in the adsorption experiments, the adsorption capacity of protein DSR A can reach 182.3 mg/g in the condition at 10 mg/L U(VI) and pH = 6. The Langmuir isotherm model and the pseudo-first-order kinetic equation were used to better describe the absorption process. We can confirm that Na, Sr and K, these three metal ions have less effect on the enrichment of U(VI) by protein DSR A compared with other common cations. Besides, we can educe some mechanisms for the removal of U (VI) by protein DSR A from the results of FTIR, SEM-EDS, XPS (binding energy = 2.0 ~ 4.0ke V), MAP and XRD analysis before and after adsorption. This work has demonstrated the great potential of genetic engineering and biological methods in dealing with environmental heavy ion pollution.
通过生物方法从环境中摄取 U(VI) 是一种环保且高效的方法。在这项工作中，我们通过基因工程获得并分离出了蛋白 DSR A，然后评估了其从废水中吸收 U(VI) 的能力和机制。结果表明，蛋白 DSR A 可以精确识别、富集和去除模拟废水中的铀酰离子。在吸附实验中证明了其巨大的去除潜力，在 10mg/L U(VI)和 pH=6 的条件下，蛋白 DSR A 的吸附容量可达 182.3mg/g。Langmuir 等温模型和拟一级动力学方程被用来更好地描述吸附过程。我们可以确认，与其他常见阳离子相比，Na、Sr 和 K 这三种金属离子对蛋白 DSR A 富集 U(VI)的影响较小。此外，我们可以从吸附前后的 FTIR、SEM-EDS、XPS（结合能=2.0~4.0keV）、MAP 和 XRD 分析结果中推导出蛋白 DSR A 去除 U(VI)的一些机制。这项工作证明了遗传工程和生物方法在处理环境重离子污染方面的巨大潜力。
