Kandeda Antoine Kavaye, Nguedia Danide, Djeuzong Etienne, Kouamouo Jonas, Dimo Théophile
Department of Animal Biology and Physiology, University of Yaoundé I, Yaoundé, P.O. Box 812, Cameroon.
Department of Pharmacy, University of the Mountains, Bangangté, P.O. Box 208, Cameroon.
Metab Brain Dis. 2022 Dec;37(8):2995-3009. doi: 10.1007/s11011-022-01050-0. Epub 2022 Aug 4.
Pharmacological treatments against Alzheimer disease provide only symptomatic relief and are associated with numerous side effects. Previous studies showed that a concoction of Ziziphus jujuba leaves possesses anti-amnesic effects in scopolamine-treated rats. More recently, an aqueous macerate of Z. jujuba leaves has been shown to reduce short-term memory impairment in D-galactose-treated rats. However, no study on the effect of an aqueous macerate of Z. jujuba on long-term memory impairment was performed. Therefore, this study evaluates the effect of an aqueous macerate of Z. jujuba on long-term spatial memory impairment in D-galactose-treated rats. Long-term spatial memory impairment was induced in rats by administering D-galactose (350 mg/kg/day, s.c.), once dailyfor 21 days. On the 22nd day, the integrity of this memory was assessed using the Morris water maze task. Rats that developed memory impairment were treated with tacrine (10 mg/kg, p.o.), or aspirin (20 mg/kg, p.o.), or extract (41.5, 83, and 166 mg/kg, p.o.), once daily, for 14 days. At the end of the treatment, memory impairment was once more assessed using the same paradigm. Animals were then euthanized, and some pro-inflammatory cytokine markers were analyzed in the hippocampus or blood. The extract at all doses significantly reduced the latency to attain the platforming of the water maze test. The extract (83 mg/kg) also increased the time spent in the target quadrant during the retention phase. The extract markedly reduced the concentration of pro-inflammatory cytokine markers in the hippocampus and blood. Together, these results suggest that this aqueous extract Z. jujuba reduces long-term spatial memory impairment. This effect may be mediated in part by its anti-inflammatory activity.
治疗阿尔茨海默病的药物仅能缓解症状，且伴有诸多副作用。先前的研究表明，酸枣叶 concoction 对东莨菪碱处理的大鼠具有抗遗忘作用。最近，酸枣叶水浸软物已被证明可减轻 D - 半乳糖处理大鼠的短期记忆损伤。然而，尚未有关于酸枣叶水浸软物对长期记忆损伤影响的研究。因此，本研究评估酸枣叶水浸软物对 D - 半乳糖处理大鼠长期空间记忆损伤的影响。通过皮下注射 D - 半乳糖（350 mg/kg/天）诱导大鼠长期空间记忆损伤，持续 21 天，每天 1 次。在第 22 天，使用莫里斯水迷宫任务评估这种记忆的完整性。出现记忆损伤的大鼠分别用他克林（10 mg/kg，口服）、阿司匹林（20 mg/kg，口服）或提取物（41.5、83 和 166 mg/kg，口服）治疗，每天 1 次，持续 14 天。治疗结束时，使用相同范式再次评估记忆损伤。然后对动物实施安乐死，并分析海马体或血液中的一些促炎细胞因子标志物。所有剂量的提取物均显著缩短了水迷宫试验中到达平台的潜伏期。提取物（83 mg/kg）还增加了在保持期目标象限停留的时间。提取物显著降低了海马体和血液中促炎细胞因子标志物的浓度。这些结果共同表明，这种酸枣叶水提取物可减轻长期空间记忆损伤。这种作用可能部分由其抗炎活性介导。
