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马属动物甲状旁腺激素的放射免疫测定法

Radioimmunoassay for parathyroid hormone in equids.

作者信息

Roussel A J, Lin Y C, Strait J R, Modransky P D

出版信息

Am J Vet Res. 1987 Apr;48(4):586-9.

PMID:3592356
Abstract

Radioimmunoassay for parathyroid hormone (PTH) in equids was performed on blood samples from healthy equids and equids with hypercalcemia and hypophosphatemia. The assay was validated for equine carboxy-terminal PTH. Manipulation of serum ionized Ca in healthy equids by infusing Na2 EDTA and CaCl2 produced an expected increase and decrease, respectively, in measurable immunoreactive PTH. Intra-assay and interassay coefficients of variation were 2.6% and 11.7%, respectively. The range of PTH valves for healthy mature horse mares and geldings maintained on pasture was less than 0.27 ng/ml to 0.92 ng/ml and for horse colts fed grain was 0.61 to 1.25 ng/ml. Serum PTH values were measured on 2 equine patients with hypercalcemia, 1 pony with primary hyperparathyroidism and 1 horse with pseudohyperparathyroidism. Both patients had increased serum PTH values.

摘要

对健康马以及患有高钙血症和低磷血症的马的血样进行了马甲状旁腺激素(PTH)的放射免疫分析。该分析方法已针对马的羧基末端PTH进行了验证。通过输注Na2 EDTA和CaCl2对健康马的血清离子钙进行调控,可分别使可测量的免疫反应性PTH出现预期的升高和降低。批内和批间变异系数分别为2.6%和11.7%。在牧场饲养的健康成年母马和去势公马的PTH值范围为小于0.27 ng/ml至0.92 ng/ml,而喂食谷物的马驹的PTH值范围为0.61至1.25 ng/ml。对2例患有高钙血症的马属动物患者、1例患有原发性甲状旁腺功能亢进的小马和1例患有假性甲状旁腺功能亢进的马测量了血清PTH值。两名患者的血清PTH值均升高。

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