LeaMaster B R, Shen D T, Gorham J R, Leathers C W, Wells H D
Am J Vet Res. 1987 May;48(5):869-72.
The efficacy of a Corynebacterium pseudotuberculosis bacterin to protect sheep immunologically against development of caseous lymphadenitis was evaluated in controlled challenge-exposure experiments. Sixty-three mixed-breed, white-faced lambs were used. The lambs were 10 to 12 weeks old and were randomly assigned to 3 groups (21 lambs/group). Group 1 was vaccinated once, using 2 ml of a C pseudotuberculosis bacterin (given subcutaneously) in the right axillary region at the beginning of the study. Group 2 was vaccinated twice; the 1st vaccination was given at the same time that lambs in group 1 were vaccinated and the 2nd vaccination was given 4 weeks later. Group 3 (nonvaccinated controls) was given physiologic saline solution (2 ml, subcutaneously). Each lamb was challenge exposed (ie, given 2 ml of live Corynebacterium pseudotuberculosis inoculum [6 X 10(6) colony-forming units/ml], subcutaneously at 4 different sites) during the 20th week of the study. All lambs were killed and necropsied during week 33. The mean number of abscesses per lamb was 7 for group 1, 4 for group 2, and 32 for group 3. Significant differences in the size of the abscesses were not found between the groups. Results of the study indicated that the vaccine provided immunologic protection of lambs against challenge exposure to Corynebacterium pseudotuberculosis.
在对照的攻毒暴露实验中，评估了假结核棒状杆菌菌苗对绵羊进行免疫保护以预防干酪性淋巴结炎发生的效果。使用了63只混种白面羔羊。这些羔羊10至12周龄，随机分为3组（每组21只羔羊）。第1组在研究开始时于右腋窝区域皮下接种2毫升假结核棒状杆菌菌苗，接种一次。第2组接种两次；第1次接种与第1组羔羊同时进行，第2次接种在4周后进行。第3组（未接种对照）皮下注射生理盐水（2毫升）。在研究的第20周，每只羔羊均接受攻毒暴露（即于4个不同部位皮下注射2毫升活的假结核棒状杆菌接种物[6×10(6)菌落形成单位/毫升]）。在第33周时，所有羔羊均被处死并进行尸检。第1组每只羔羊的平均脓肿数为7个，第2组为4个，第3组为32个。各组之间脓肿大小未发现显著差异。研究结果表明，该疫苗为羔羊提供了针对假结核棒状杆菌攻毒暴露的免疫保护。
